|
|
| (9 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:3cnz.jpg|left|200px]] | |
| | | | |
| - | <!--
| + | ==Structural dynamics of the microtubule binding and regulatory elements in the kinesin-like calmodulin binding protein== |
| - | The line below this paragraph, containing "STRUCTURE_3cnz", creates the "Structure Box" on the page.
| + | <StructureSection load='3cnz' size='340' side='right'caption='[[3cnz]], [[Resolution|resolution]] 2.90Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[3cnz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CNZ FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | {{STRUCTURE_3cnz| PDB=3cnz | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cnz OCA], [https://pdbe.org/3cnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cnz RCSB], [https://www.ebi.ac.uk/pdbsum/3cnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cnz ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''Structural dynamics of the microtubule binding and regulatory elements in the kinesin-like calmodulin binding protein'''
| + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/Q41460_SOLTU Q41460_SOLTU] |
| - | | + | == Evolutionary Conservation == |
| - | ==Overview== | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | Kinesins are molecular motors that power cell division and transport of various proteins and organelles. Their motor activity is driven by ATP hydrolysis and depends on interactions with microtubule tracks. Essential steps in kinesin movement rely on controlled alternate binding to and detaching from the microtubules. The conformational changes in the kinesin motors induced by nucleotide and microtubule binding are coordinated by structural elements within their motor domains. Loop L11 of the kinesin motor domain interacts with the microtubule and is implicated in both microtubule binding and sensing nucleotide bound to the active site of kinesin. Consistent with its proposed role as a microtubule sensor, loop L11 is rarely seen in crystal structures of unattached kinesins. Here, we report four structures of a regulated plant kinesin, the kinesin-like calmodulin binding protein (KCBP), determined by X-ray crystallography. Although all structures reveal the kinesin motor in the ATP-like conformation, its loop L11 is observed in different conformational states, both ordered and disordered. When structured, loop L11 adds three additional helical turns to the N-terminal part of the following helix alpha4. Although interactions with protein neighbors in the crystal support the ordering of loop L11, its observed conformation suggests the conformation for loop L11 in the microtubule-bound kinesin. Variations in the positions of other features of these kinesins were observed. A critical regulatory element of this kinesin, the calmodulin binding helix positioned at the C-terminus of the motor domain, is thought to confer negative regulation of KCBP. Calmodulin binds to this helix and inserts itself between the motor and the microtubule. Comparison of five independent structures of KCBP shows that the positioning of the calmodulin binding helix is not decided by crystal packing forces but is determined by the conformational state of the motor. The observed variations in the position of the calmodulin binding helix fit the regulatory mechanism previously proposed for this kinesin motor.
| + | Check<jmol> |
| - | | + | <jmolCheckbox> |
| - | ==About this Structure== | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/3cnz_consurf.spt"</scriptWhenChecked> |
| - | 3CNZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNZ OCA].
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | ==Reference== | + | </jmolCheckbox> |
| - | Structural dynamics of the microtubule binding and regulatory elements in the kinesin-like calmodulin binding protein., Vinogradova MV, Malanina GG, Reddy VS, Reddy AS, Fletterick RJ, J Struct Biol. 2008 May 5;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18513992 18513992]
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cnz ConSurf]. |
| - | [[Category: Single protein]] | + | <div style="clear:both"></div> |
| | + | __TOC__ |
| | + | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Solanum tuberosum]] | | [[Category: Solanum tuberosum]] |
| - | [[Category: Fletterick, R J.]] | + | [[Category: Fletterick RJ]] |
| - | [[Category: Malanina, G G.]] | + | [[Category: Malanina GG]] |
| - | [[Category: Reddy, A S.N.]] | + | [[Category: Reddy ASN]] |
| - | [[Category: Reddy, V.]] | + | [[Category: Reddy V]] |
| - | [[Category: Vinogradova, M V.]] | + | [[Category: Vinogradova MV]] |
| - | [[Category: Atp-binding]]
| + | |
| - | [[Category: Kinesin]]
| + | |
| - | [[Category: Loop l11]]
| + | |
| - | [[Category: Microtubule]]
| + | |
| - | [[Category: Motor]]
| + | |
| - | [[Category: Motor protein]]
| + | |
| - | [[Category: Nucleotide-binding]]
| + | |
| - | [[Category: Switch ii]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:10:58 2008''
| + | |
