2o9u

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[[Image:2o9u.jpg|left|200px]]
 
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==Monellin (MNEI) at 1.15 resolution==
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The line below this paragraph, containing "STRUCTURE_2o9u", creates the "Structure Box" on the page.
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<StructureSection load='2o9u' size='340' side='right'caption='[[2o9u]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2o9u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O9U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2o9u| PDB=2o9u | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o9u OCA], [https://pdbe.org/2o9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o9u RCSB], [https://www.ebi.ac.uk/pdbsum/2o9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o9u ProSAT]</span></td></tr>
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</table>
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'''Monellin (MNEI) at 1.15 resolution'''
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== Function ==
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[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.
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<div style="background-color:#fffaf0;">
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==Overview==
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== Publication Abstract from PubMed ==
The X-ray crystal structure of a single-chain monellin protein (MNEI) has been determined at 1.15 A resolution. The model was refined to convergence employing anisotropic displacement parameters and riding H atoms to produce a final model with R(work) and R(free) values of 0.132 and 0.162, respectively. The crystal contains a single MNEI protein in the asymmetric unit and unusually lacks the dimer interface observed in all previous crystal structures of monellin and its single-chain derivatives. The high resolution allowed a more detailed view of MNEI than previously possible, with 38 of the 96 residues modelled with alternative side-chain conformations, including four core residues Thr12, Cys41, Leu62 and Ile75. Four stably bound negative ions were also located, providing new insight into potential electrostatic interactions of MNEI with the largely negatively charged surface of the sweet taste receptor T1R2-T1R3.
The X-ray crystal structure of a single-chain monellin protein (MNEI) has been determined at 1.15 A resolution. The model was refined to convergence employing anisotropic displacement parameters and riding H atoms to produce a final model with R(work) and R(free) values of 0.132 and 0.162, respectively. The crystal contains a single MNEI protein in the asymmetric unit and unusually lacks the dimer interface observed in all previous crystal structures of monellin and its single-chain derivatives. The high resolution allowed a more detailed view of MNEI than previously possible, with 38 of the 96 residues modelled with alternative side-chain conformations, including four core residues Thr12, Cys41, Leu62 and Ile75. Four stably bound negative ions were also located, providing new insight into potential electrostatic interactions of MNEI with the largely negatively charged surface of the sweet taste receptor T1R2-T1R3.
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==About this Structure==
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Monellin (MNEI) at 1.15 A resolution.,Hobbs JR, Munger SD, Conn GL Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):162-7. Epub 2007 Feb 13. PMID:17329805<ref>PMID:17329805</ref>
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2O9U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O9U OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Monellin (MNEI) at 1.15 A resolution., Hobbs JR, Munger SD, Conn GL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt, 3):162-7. Epub 2007 Feb 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17329805 17329805]
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</div>
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<div class="pdbe-citations 2o9u" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Dioscoreophyllum cumminsii]]
[[Category: Dioscoreophyllum cumminsii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Conn, G L.]]
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[[Category: Conn GL]]
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[[Category: Hobbs, J R.]]
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[[Category: Hobbs JR]]
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[[Category: Munger, S D.]]
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[[Category: Munger SD]]
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[[Category: 3-10 polyproline ii helix]]
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[[Category: Alternative conformation]]
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[[Category: Plant protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:16:24 2008''
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Current revision

Monellin (MNEI) at 1.15 resolution

PDB ID 2o9u

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