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1cgu

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(New page: 200px<br /><applet load="1cgu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cgu, resolution 2.5&Aring;" /> '''CATALYTIC CENTER OF C...)
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[[Image:1cgu.jpg|left|200px]]<br /><applet load="1cgu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cgu, resolution 2.5&Aring;" />
 
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'''CATALYTIC CENTER OF CYCLODEXTRIN GLYCOSYLTRANSFERASE DERIVED FROM X-RAY STRUCTURE ANALYSIS COMBINED WITH SITE-DIRECTED MUTAGENESIS'''<br />
 
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==Overview==
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==CATALYTIC CENTER OF CYCLODEXTRIN GLYCOSYLTRANSFERASE DERIVED FROM X-RAY STRUCTURE ANALYSIS COMBINED WITH SITE-DIRECTED MUTAGENESIS==
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An X-ray structure analysis of a crystal of mutant Asp229----Ala of, cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19), that had been shortly exposed to beta-cyclodextrin showed density, corresponding to a maltose bound at the catalytic center. The crystal, structure was refined to an R-factor of 18.7% at 2.5-A resolution. The, catalytic center is defined by homology with the structurally known, alpha-amylases and by the observation that mutants Asp229----Ala and, Asp328----Ala are almost inactive. By model building, the density-defined, maltose was extended to a full beta-cyclodextrin, which then indicated the, general locations of seven subsites for glucosyl units. The catalytically, competent residues Asp229, Glu257, and Asp328 are at the reducing end of, the density-defined maltose. In the unligated wild-type structure, Glu257, and Asp328 form a 2.6-A hydrogen bond between their carboxylates in an, arrangement that resembles those of the catalytically competent, carboxylates in acid proteases. Presumably, the first catalytic step is an, attack of the proton between Glu257 and Asp328 on the oxygen of the, glycosidic bond.
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<StructureSection load='1cgu' size='340' side='right'caption='[[1cgu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cgu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CGU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgu OCA], [https://pdbe.org/1cgu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cgu RCSB], [https://www.ebi.ac.uk/pdbsum/1cgu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDGT1_NIACI CDGT1_NIACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgu ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CGU OCA].
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Catalytic center of cyclodextrin glycosyltransferase derived from X-ray structure analysis combined with site-directed mutagenesis., Klein C, Hollender J, Bender H, Schulz GE, Biochemistry. 1992 Sep 22;31(37):8740-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1390660 1390660]
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[[Category: Large Structures]]
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[[Category: Bacillus circulans]]
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[[Category: Niallia circulans]]
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[[Category: Cyclomaltodextrin glucanotransferase]]
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[[Category: Bender H]]
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[[Category: Single protein]]
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[[Category: Hollender J]]
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[[Category: Bender, H.]]
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[[Category: Klein C]]
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[[Category: Hollender, J.]]
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[[Category: Schulz GE]]
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[[Category: Klein, C.]]
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[[Category: Schulz, G.E.]]
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[[Category: CA]]
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[[Category: glycosyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:26:18 2007''
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Current revision

CATALYTIC CENTER OF CYCLODEXTRIN GLYCOSYLTRANSFERASE DERIVED FROM X-RAY STRUCTURE ANALYSIS COMBINED WITH SITE-DIRECTED MUTAGENESIS

PDB ID 1cgu

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