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1cma

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(New page: 200px<br /><applet load="1cma" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cma, resolution 2.800&Aring;" /> '''MET REPRESSOR/DNA C...)
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[[Image:1cma.jpg|left|200px]]<br /><applet load="1cma" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cma, resolution 2.800&Aring;" />
 
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'''MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE'''<br />
 
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==Overview==
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==MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE==
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The crystal structure of the met repressor-operator complex shows two, dimeric repressor molecules bound to adjacent sites 8 base pairs apart on, an 18-base-pair DNA fragment. Sequence specificity is achieved by, insertion of double-stranded antiparallel protein beta-ribbons into the, major groove of B-form DNA, with direct hydrogen-bonding between, amino-acid side chains and the base pairs. The repressor also recognizes, sequence-dependent distortion or flexibility of the operator phosphate, backbone, conferring specificity even for inaccessible base pairs.
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<StructureSection load='1cma' size='340' side='right'caption='[[1cma]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cma]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CMA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cma OCA], [https://pdbe.org/1cma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cma RCSB], [https://www.ebi.ac.uk/pdbsum/1cma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cma ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/METJ_ECOLI METJ_ECOLI] This regulatory protein, when combined with SAM (S-adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis. It is also autoregulated.[HAMAP-Rule:MF_00744]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/1cma_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cma ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CMA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SAM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CMA OCA].
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*[[Met repressor|Met repressor]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the met repressor-operator complex at 2.8 A resolution reveals DNA recognition by beta-strands., Somers WS, Phillips SE, Nature. 1992 Oct 1;359(6394):387-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1406951 1406951]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Phillips, S.E.V.]]
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[[Category: Phillips SEV]]
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[[Category: Somers, W.S.]]
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[[Category: Somers WS]]
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[[Category: SAM]]
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[[Category: double helix]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:34:40 2007''
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Current revision

MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE

PDB ID 1cma

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