1cnz

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(New page: 200px<br /><applet load="1cnz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cnz, resolution 1.76&Aring;" /> '''3-ISOPROPYLMALATE DE...)
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[[Image:1cnz.gif|left|200px]]<br /><applet load="1cnz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cnz, resolution 1.76&Aring;" />
 
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'''3-ISOPROPYLMALATE DEHYDROGENASE (IPMDH) FROM SALMONELLA TYPHIMURIUM'''<br />
 
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==Overview==
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==3-ISOPROPYLMALATE DEHYDROGENASE (IPMDH) FROM SALMONELLA TYPHIMURIUM==
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The basis of protein stability has been investigated by the structural, comparison of themophilic enzymes with their mesophilic counterparts. A, number of characteristics have been found that can contribute to the, stabilization of thermophilic proteins, but no one is uniquely capable of, imparting thermostability. The crystal structure of 3-isopropylmalate, dehydrogenase (IPMDH) from the mesophiles Escherichia coli and Salmonella, typhimurium have been determined by the method of molecular replacement, using the known structure of the homologous Thermus thermophilus enzyme., The structure of the E. coli enzyme was refined at a resolution of 2.1 A, to an R-factor of 17.3%, that of the S. typhimurium enzyme at 1.7 A, resolution to an R-factor of 19.8%. The three structures were compared to, elucidate the basis of the higher thermostability of the T. thermophilus, enzyme. A mutant that created a cavity in the hydrophobic core of the, thermophilic enzyme was designed to investigate the importance of packing, density for thermostability. The structure of this mutant was analyzed., The main stabilizing features in the thermophilic enzyme are an increased, number of salt bridges, additional hydrogen bonds, a proportionately, larger and more hydrophobic subunit interface, shortened N and C termini, and a larger number of proline residues. The mutation in the hydrophobic, core of T. thermophilus IPMDH resulted in a cavity of 32 A3, but no, significant effect on the activity and thermostability of the mutant was, observed.
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<StructureSection load='1cnz' size='340' side='right'caption='[[1cnz]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cnz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CNZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cnz OCA], [https://pdbe.org/1cnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cnz RCSB], [https://www.ebi.ac.uk/pdbsum/1cnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cnz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEU3_SALTY LEU3_SALTY] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01033]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/1cnz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cnz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CNZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with MN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CNZ OCA].
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*[[Isopropylmalate dehydrogenase|Isopropylmalate dehydrogenase]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilus., Wallon G, Kryger G, Lovett ST, Oshima T, Ringe D, Petsko GA, J Mol Biol. 1997 Mar 14;266(5):1016-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9086278 9086278]
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[[Category: Large Structures]]
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[[Category: Salmonella typhimurium]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Single protein]]
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[[Category: Kryger G]]
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[[Category: Kryger, G.]]
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[[Category: Lovett ST]]
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[[Category: Lovett, S.T.]]
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[[Category: Oshima T]]
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[[Category: Oshima, T.]]
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[[Category: Petsko GA]]
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[[Category: Petsko, G.A.]]
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[[Category: Ringe D]]
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[[Category: Ringe, D.]]
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[[Category: Wallon G]]
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[[Category: Wallon, G.]]
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[[Category: MN]]
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[[Category: SO4]]
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[[Category: dehydrogenase]]
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[[Category: leucine biosynthetic pathway]]
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[[Category: nad-dependant enzyme]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:36:45 2007''
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Current revision

3-ISOPROPYLMALATE DEHYDROGENASE (IPMDH) FROM SALMONELLA TYPHIMURIUM

PDB ID 1cnz

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