1crw

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(New page: 200px<br /><applet load="1crw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1crw, resolution 2.0&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1crw.jpg|left|200px]]<br /><applet load="1crw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1crw, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF APO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PALINURUS VERSICOLOR AT 2.0A RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF APO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PALINURUS VERSICOLOR AT 2.0A RESOLUTION==
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d-Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) shows cooperative, properties for binding coenzymes. The structure of apo-GAPDH from, Palinurus versicolor has been solved at 2.0 A resolution by X-ray, crystallography. The final model gives a crystallographic R factor of, 0.178 in the resolution range 8 to 2 A. The structural comparison with, holo-GAPDH from the same species reveals a conformational change induced, by coenzyme binding similar to that observed in Bacillus, stearothermophilus GAPDH but to a lesser extent. The differences in, magnitude during the apo-holo transition between these two enzymes were, analyzed with respect to the change of the amino acid composition in the, coenzyme binding pocket. In the crystalline state of apo-GAPDH, the, overall structures of the subunits are similar to each other; however, significant differences in temperature factors and minor differences in, domain rotation upon coenzyme binding were observed for different, subunits. These structural features are discussed in relation to the, environmental asymmetry of crystallographically independent subunits.
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<StructureSection load='1crw' size='340' side='right'caption='[[1crw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1crw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Panulirus_versicolor Panulirus versicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CRW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1crw OCA], [https://pdbe.org/1crw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1crw RCSB], [https://www.ebi.ac.uk/pdbsum/1crw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1crw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/G3P_PANVR G3P_PANVR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1crw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1crw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CRW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Palinurus_versicolor Palinurus versicolor]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CRW OCA].
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*[[Aldehyde dehydrogenase 3D structures|Aldehyde dehydrogenase 3D structures]]
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*[[Glyceraldehyde-3-phosphate dehydrogenase 3D structures|Glyceraldehyde-3-phosphate dehydrogenase 3D structures]]
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==Reference==
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__TOC__
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Structure of apo-glyceraldehyde-3-phosphate dehydrogenase from Palinurus versicolor., Shen YQ, Li J, Song SY, Lin ZJ, J Struct Biol. 2000 May;130(1):1-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10806086 10806086]
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</StructureSection>
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[[Category: Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]]
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[[Category: Large Structures]]
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[[Category: Palinurus versicolor]]
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[[Category: Panulirus versicolor]]
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[[Category: Single protein]]
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[[Category: Li J]]
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[[Category: Li, J.]]
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[[Category: Lin Z]]
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[[Category: Lin, Z.]]
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[[Category: Shen Y]]
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[[Category: Shen, Y.]]
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[[Category: Song S]]
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[[Category: Song, S.]]
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[[Category: free-nad gapdh]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:42:43 2007''
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Current revision

CRYSTAL STRUCTURE OF APO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PALINURUS VERSICOLOR AT 2.0A RESOLUTION

PDB ID 1crw

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