1cx4

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(New page: 200px<br /><applet load="1cx4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cx4, resolution 2.45&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1cx4.jpg|left|200px]]<br /><applet load="1cx4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cx4, resolution 2.45&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A DELETION MUTANT OF THE TYPE II BETA REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A DELETION MUTANT OF THE TYPE II BETA REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE==
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BACKGROUND: Cyclic AMP binding domains possess common structural features, yet are diversely coupled to different signaling modules. Each cAMP, binding domain receives and transmits a cAMP signal; however, the, signaling networks differ even within the same family of regulatory, proteins as evidenced by the long-standing biochemical and physiological, differences between type I and type II regulatory subunits of, cAMP-dependent protein kinase. RESULTS: We report the first type II, regulatory subunit crystal structure, which we determined to 2.45 A, resolution and refined to an R factor of 0.176 with a free R factor of, 0.198. This new structure of the type II beta regulatory subunit of, cAMP-dependent protein kinase demonstrates that the relative orientations, of the two tandem cAMP binding domains are very different in the type II, beta as compared to the type I alpha regulatory subunit. Each structural, unit for binding cAMP contains the highly conserved phosphate binding, cassette that can be considered the "signature" motif of cAMP binding, domains. This motif is coupled to nonconserved regions that link the cAMP, signal to diverse structural and functional modules. CONCLUSIONS: Both the, diversity and similarity of cAMP binding sites are demonstrated by this, new type II regulatory subunit structure. The structure represents an, intramolecular paradigm for the cooperative triad that links two cAMP, binding sites through a domain interface to the catalytic subunit of, cAMP-dependent protein kinase. The domain interface surface is created by, the binding of only one cAMP molecule and is enabled by amino acid, sequence variability within the peptide chain that tethers the two domains, together.
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<StructureSection load='1cx4' size='340' side='right'caption='[[1cx4]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cx4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The August 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''cAMP-dependent Protein Kinase (PKA)'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_8 10.2210/rcsb_pdb/mom_2012_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CX4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cx4 OCA], [https://pdbe.org/1cx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cx4 RCSB], [https://www.ebi.ac.uk/pdbsum/1cx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cx4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KAP3_RAT KAP3_RAT] Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/1cx4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cx4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CX4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CMP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CX4 OCA].
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*[[CAMP-dependent protein kinase 3D structures|CAMP-dependent protein kinase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Molecular basis for regulatory subunit diversity in cAMP-dependent protein kinase: crystal structure of the type II beta regulatory subunit., Diller TC, Madhusudan, Xuong NH, Taylor SS, Structure. 2001 Jan 10;9(1):73-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11342137 11342137]
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[[Category: Large Structures]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Diller TC]]
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[[Category: Diller, T.C.]]
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[[Category: Taylor SS]]
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[[Category: Taylor, S.S.]]
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[[Category: Xuong NH]]
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[[Category: Xuong, N.H.]]
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[[Category: CMP]]
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[[Category: beta barrel]]
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[[Category: camp-binding]]
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[[Category: camp-dependent protein kinase]]
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[[Category: regulatory subunit]]
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[[Category: signaling protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:49:10 2007''
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Current revision

CRYSTAL STRUCTURE OF A DELETION MUTANT OF THE TYPE II BETA REGULATORY SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE

PDB ID 1cx4

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