1cxi

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(New page: 200px<br /><applet load="1cxi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cxi, resolution 2.2&Aring;" /> '''WILD-TYPE CGTASE FROM...)
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[[Image:1cxi.gif|left|200px]]<br /><applet load="1cxi" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cxi, resolution 2.2&Aring;" />
 
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'''WILD-TYPE CGTASE FROM BACILLUS CIRCULANS STRAIN 251 AT 120 K AND PH 7.55'''<br />
 
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==Overview==
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==WILD-TYPE CGTASE FROM BACILLUS CIRCULANS STRAIN 251 AT 120 K AND PH 7.55==
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Asp-229, Glu-257, and Asp-328 constitute the catalytic residues in, cyclodextrin glycosyl transferase from Bacillus circulans strain 251. Via, site-directed mutagenesis constructed D229N, E257Q, and D328N mutant, proteins showed a 4,000-60,000-fold reduction of cyclization activity. A, D229N/E257Q double mutant showed a 700,000-fold reduction and was, crystallized for use in soaking experiments with alpha-cyclodextrin., Crystal structures were determined of wild type CGTase soaked at elevated, pH with alpha-cyclodextrin (resolution, 2.1 A) and maltoheptaose (2.4 A)., In addition, structures at cryogenic temperature were solved of the, unliganded enzyme (2.2 A) and of the D229N/E257Q mutant after soaking with, alpha-cyclodextrin (2.6 A). In the crystals soaked in alpha-cyclodextrin, and maltoheptaose, a maltotetraose molecule is observed to bind in the, active site. Residue 229 is at hydrogen bonding distance from the C-6, hydroxyl group of the sugar, which after cleavage will contain the new, reducing end. In the D229N/E257Q double mutant structure, two, alpha-cyclodextrins are observed to replace two maltoses at the E-domain, thus providing structural information on product inhibition via binding to, the enzyme's raw starch binding domain.
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<StructureSection load='1cxi' size='340' side='right'caption='[[1cxi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cxi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CXI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxi OCA], [https://pdbe.org/1cxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cxi RCSB], [https://www.ebi.ac.uk/pdbsum/1cxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cxi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDGT2_NIACI CDGT2_NIACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cx/1cxi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cxi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CXI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with MAL and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CXI OCA].
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystallographic studies of the interaction of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 with natural substrates and products., Knegtel RM, Strokopytov B, Penninga D, Faber OG, Rozeboom HJ, Kalk KH, Dijkhuizen L, Dijkstra BW, J Biol Chem. 1995 Dec 8;270(49):29256-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7493956 7493956]
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[[Category: Large Structures]]
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[[Category: Bacillus circulans]]
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[[Category: Niallia circulans]]
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[[Category: Cyclomaltodextrin glucanotransferase]]
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[[Category: Dijkstra BW]]
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[[Category: Single protein]]
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[[Category: Knegtel RMA]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Strokopytov BV]]
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[[Category: Knegtel, R.M.A.]]
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[[Category: Strokopytov, B.V.]]
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[[Category: CA]]
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[[Category: MAL]]
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[[Category: glycosyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:49:44 2007''
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Current revision

WILD-TYPE CGTASE FROM BACILLUS CIRCULANS STRAIN 251 AT 120 K AND PH 7.55

PDB ID 1cxi

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