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1cz7

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(New page: 200px<br /><applet load="1cz7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cz7, resolution 2.9&Aring;" /> '''THE CRYSTAL STRUCTURE...)
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[[Image:1cz7.gif|left|200px]]<br /><applet load="1cz7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cz7, resolution 2.9&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS==
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BACKGROUND: The kinesin superfamily of microtubule-associated motor, proteins are important for intracellular transport and for cell division, in eukaryotes. Conventional kinesins have the motor domain at the N, terminus of the heavy chain and move towards the plus end of microtubules., The ncd protein is necessary for chromosome segregation in meiosis. It, belongs to a subfamily of kinesins that have the motor domain at the C, terminus and move towards the minus end of microtubules. RESULTS: The, crystal structure of dimeric ncd has been obtained at 2.9 A resolution, from crystals with the C222(1) space group, with two independent dimers, per asymmetric unit. The motor domains in these dimers are not related by, crystallographic symmetry and the two ncd dimers have significantly, different conformations. An alpha-helical coiled coil connects, and, interacts with, the motor domains. CONCLUSIONS: The ncd protein has a very, compact structure, largely due to extended interactions of the coiled coil, with the head domains. Despite this, we find that the overall conformation, of the ncd dimer can be rotated by as much as 10 degrees away from that of, the twofold-symmetric archetypal ncd. The crystal structures of, conventional kinesin and of ncd suggest a structural rationale for the, reversal of the direction of movement in chimeric kinesins.
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<StructureSection load='1cz7' size='340' side='right'caption='[[1cz7]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cz7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CZ7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cz7 OCA], [https://pdbe.org/1cz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cz7 RCSB], [https://www.ebi.ac.uk/pdbsum/1cz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cz7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCD_DROME NCD_DROME] NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.<ref>PMID:2146510</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cz/1cz7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cz7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CZ7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CZ7 OCA].
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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== References ==
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==Reference==
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<references/>
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The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations., Kozielski F, De Bonis S, Burmeister WP, Cohen-Addad C, Wade RH, Structure. 1999 Nov 15;7(11):1407-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10574799 10574799]
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bonis, S.De.]]
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[[Category: Burmeister W]]
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[[Category: Burmeister, W.]]
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[[Category: Cohen-Addad C]]
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[[Category: Cohen-Addad, C.]]
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[[Category: De Bonis S]]
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[[Category: Kozielski, F.K.]]
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[[Category: Kozielski FK]]
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[[Category: Wade, R.]]
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[[Category: Wade R]]
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[[Category: ADP]]
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[[Category: MG]]
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[[Category: kinesin superfamily]]
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[[Category: microtubule motors]]
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[[Category: ncd crystal structure]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:52:17 2007''
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THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS

PDB ID 1cz7

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