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1czf

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(New page: 200px<br /><applet load="1czf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1czf, resolution 1.68&Aring;" /> '''ENDO-POLYGALACTURONA...)
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[[Image:1czf.gif|left|200px]]<br /><applet load="1czf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1czf, resolution 1.68&Aring;" />
 
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'''ENDO-POLYGALACTURONASE II FROM ASPERGILLUS NIGER'''<br />
 
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==Overview==
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==ENDO-POLYGALACTURONASE II FROM ASPERGILLUS NIGER==
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Polygalacturonases specifically hydrolyze polygalacturonate, a major, constituent of plant cell wall pectin. To understand the catalytic, mechanism and substrate and product specificity of these enzymes, we have, solved the x-ray structure of endopolygalacturonase II of Aspergillus, niger and we have carried out site-directed mutagenesis studies. The, enzyme folds into a right-handed parallel beta-helix with 10 complete, turns. The beta-helix is composed of four parallel beta-sheets, and has, one very small alpha-helix near the N terminus, which shields the enzyme's, hydrophobic core. Loop regions form a cleft on the exterior of the, beta-helix. Site-directed mutagenesis of Asp(180), Asp(201), Asp(202), His(223), Arg(256), and Lys(258), which are located in this cleft, results, in a severe reduction of activity, demonstrating that these residues are, important for substrate binding and/or catalysis. The juxtaposition of the, catalytic residues differs from that normally encountered in inverting, glycosyl hydrolases. A comparison of the endopolygalacturonase II active, site with that of the P22 tailspike rhamnosidase suggests that Asp(180), and Asp(202) activate the attacking nucleophilic water molecule, while, Asp(201) protonates the glycosidic oxygen of the scissile bond.
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<StructureSection load='1czf' size='340' side='right'caption='[[1czf]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1czf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CZF FirstGlance]. <br>
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1CZF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with NAG and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CZF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1czf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1czf OCA], [https://pdbe.org/1czf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1czf RCSB], [https://www.ebi.ac.uk/pdbsum/1czf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1czf ProSAT]</span></td></tr>
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1.68-A crystal structure of endopolygalacturonase II from Aspergillus niger and identification of active site residues by site-directed mutagenesis., van Santen Y, Benen JA, Schroter KH, Kalk KH, Armand S, Visser J, Dijkstra BW, J Biol Chem. 1999 Oct 22;274(43):30474-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10521427 10521427]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGLR2_ASPNA PGLR2_ASPNA] Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cz/1czf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1czf ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Polygalacturonase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Dijkstra BW]]
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[[Category: Dijkstra, B.W.]]
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[[Category: Kalk KH]]
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[[Category: Kalk, K.H.]]
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[[Category: Van Santen Y]]
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[[Category: Santen, Y.van.]]
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[[Category: NAG]]
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[[Category: ZN]]
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[[Category: beta helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:52:43 2007''
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Current revision

ENDO-POLYGALACTURONASE II FROM ASPERGILLUS NIGER

PDB ID 1czf

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