1d0n

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(New page: 200px<br /><applet load="1d0n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d0n, resolution 2.5&Aring;" /> '''THE CRYSTAL STRUCTURE...)
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[[Image:1d0n.jpg|left|200px]]<br /><applet load="1d0n" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1d0n, resolution 2.5&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF CALCIUM-FREE EQUINE PLASMA GELSOLIN.'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF CALCIUM-FREE EQUINE PLASMA GELSOLIN.==
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The structure of gelsolin has been determined by crystallography and, comprises six structurally related domains that, in a Ca2+-free, environment, pack together to form a compact globular structure in which, the putative actin-binding sequences are not sufficiently exposed to, enable binding to occur. We propose that binding Ca2+ can release the, connections that join the N- and C-terminal halves of gelsolin, enabling, each half to bind actin relatively independently. Domain shifts are, proposed in response to Ca2+ as bases for models of how gelsolin acts to, sever, cap, or nucleate F-actin filaments. The structure also invites, discussion of polyphosphoinositide binding to segment 2 and suggests how, mutation at Asp-187 could initiate a series of events that lead to, deposition of amyloid plaques, as observed in victims of familial, amyloidosis (Finnish type).
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<StructureSection load='1d0n' size='340' side='right'caption='[[1d0n]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1d0n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D0N FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0n OCA], [https://pdbe.org/1d0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d0n RCSB], [https://www.ebi.ac.uk/pdbsum/1d0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GELS_HORSE GELS_HORSE] Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/1d0n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d0n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of gelsolin has been determined by crystallography and comprises six structurally related domains that, in a Ca2+-free environment, pack together to form a compact globular structure in which the putative actin-binding sequences are not sufficiently exposed to enable binding to occur. We propose that binding Ca2+ can release the connections that join the N- and C-terminal halves of gelsolin, enabling each half to bind actin relatively independently. Domain shifts are proposed in response to Ca2+ as bases for models of how gelsolin acts to sever, cap, or nucleate F-actin filaments. The structure also invites discussion of polyphosphoinositide binding to segment 2 and suggests how mutation at Asp-187 could initiate a series of events that lead to deposition of amyloid plaques, as observed in victims of familial amyloidosis (Finnish type).
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==About this Structure==
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The crystal structure of plasma gelsolin: implications for actin severing, capping, and nucleation.,Burtnick LD, Koepf EK, Grimes J, Jones EY, Stuart DI, McLaughlin PJ, Robinson RC Cell. 1997 Aug 22;90(4):661-70. PMID:9288746<ref>PMID:9288746</ref>
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1D0N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D0N OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of plasma gelsolin: implications for actin severing, capping, and nucleation., Burtnick LD, Koepf EK, Grimes J, Jones EY, Stuart DI, McLaughlin PJ, Robinson RC, Cell. 1997 Aug 22;90(4):661-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9288746 9288746]
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</div>
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[[Category: Equus caballus]]
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<div class="pdbe-citations 1d0n" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Burtnick, L.D.]]
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[[Category: Li, C.]]
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[[Category: Robinson, R.]]
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[[Category: actin-binding protein]]
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[[Category: mixed alpha-beta structure]]
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[[Category: protein domain packing]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:54:53 2007''
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==See Also==
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*[[Gelsolin 3D structures|Gelsolin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Equus caballus]]
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[[Category: Large Structures]]
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[[Category: Burtnick LD]]
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[[Category: Li C]]
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[[Category: Robinson R]]

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THE CRYSTAL STRUCTURE OF CALCIUM-FREE EQUINE PLASMA GELSOLIN.

PDB ID 1d0n

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