1d0q

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1d0q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d0q, resolution 1.71&Aring;" /> '''STRUCTURE OF THE ZIN...)
Current revision (06:47, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1d0q.gif|left|200px]]<br /><applet load="1d0q" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1d0q, resolution 1.71&Aring;" />
 
-
'''STRUCTURE OF THE ZINC-BINDING DOMAIN OF BACILLUS STEAROTHERMOPHILUS DNA PRIMASE'''<br />
 
-
==Overview==
+
==STRUCTURE OF THE ZINC-BINDING DOMAIN OF BACILLUS STEAROTHERMOPHILUS DNA PRIMASE==
-
BACKGROUND: DNA primases catalyse the synthesis of the short RNA primers, that are required for DNA replication by DNA polymerases. Primases, comprise three functional domains: a zinc-binding domain that is, responsible for template recognition, a polymerase domain, and a domain, that interacts with the replicative helicase, DnaB. RESULTS: We present, the crystal structure of the zinc-binding domain of DNA primase from, Bacillus stearothermophilus, determined at 1.7 A resolution. This is the, first high-resolution structural information about any DNA primase. A, model is discussed for the interaction of this domain with the, single-stranded DNA template. CONCLUSIONS: The structure of the DNA, primase zinc-binding domain confirms that the protein belongs to the zinc, ribbon subfamily. Structural comparison with other nucleic acid binding, proteins suggests that the beta sheet of primase is likely to be the, DNA-binding surface, with conserved residues on this surface being, involved in the binding and recognition of DNA.
+
<StructureSection load='1d0q' size='340' side='right'caption='[[1d0q]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1d0q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D0Q FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d0q OCA], [https://pdbe.org/1d0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d0q RCSB], [https://www.ebi.ac.uk/pdbsum/1d0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d0q ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DNAG_GEOSE DNAG_GEOSE] RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.[HAMAP-Rule:MF_00974]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/1d0q_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d0q ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1D0Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D0Q OCA].
+
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure of the zinc-binding domain of Bacillus stearothermophilus DNA primase., Pan H, Wigley DB, Structure. 2000 Mar 15;8(3):231-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10745010 10745010]
+
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Pan, H.]]
+
[[Category: Pan H]]
-
[[Category: Wigley, D.B.]]
+
[[Category: Wigley DB]]
-
[[Category: ZN]]
+
-
[[Category: dna primase]]
+
-
[[Category: protein]]
+
-
[[Category: zinc-binding motif]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:55:02 2007''
+

Current revision

STRUCTURE OF THE ZINC-BINDING DOMAIN OF BACILLUS STEAROTHERMOPHILUS DNA PRIMASE

PDB ID 1d0q

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1d0q"
Personal tools