This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1d2p

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1d2p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d2p, resolution 2.50&Aring;" /> '''CRYSTAL STRUCTURE OF...)
Current revision (06:48, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1d2p.jpg|left|200px]]<br /><applet load="1d2p" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1d2p, resolution 2.50&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF TWO B REPEAT UNITS (B1B2) OF THE COLLAGEN BINDING PROTEIN (CNA) OF STAPHYLOCOCCUS AUREUS'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF TWO B REPEAT UNITS (B1B2) OF THE COLLAGEN BINDING PROTEIN (CNA) OF STAPHYLOCOCCUS AUREUS==
-
BACKGORUND: The Staphylococcus aureus collagen-binding protein Cna, mediates bacterial adherence to collagen. The primary sequence of Cna has, a non-repetitive collagen-binding A region, followed by the repetitive B, region. The B region has one to four 23 kDa repeat units (B(1)-B(4)), depending on the strain of origin. The affinity of the A region for, collagen is independent of the B region. However, the B repeat units have, been suggested to serve as a 'stalk' that projects the A region from the, bacterial surface and thus facilitate bacterial adherence to collagen. To, understand the biological role of these B-region repeats we determined, their three-dimensional structure. RESULTS: B(1) has two domains (D(1) and, D(2)) placed side-by-side. D(1) and D(2) have similar secondary structure, and exhibit a unique fold that resembles but is the inverse of the, immunoglobulin-like (IgG-like) domains. Comparison with similar, immunoglobulin superfamily (IgSF) structures shows novel packing, arrangements between the D(1) and D(2) domains. In the B(1)B(2) crystal, structure, an omission of a single glycine residue in the D(2)-D(3) linker, loop, compared to the D(1)-D(2) and D(3)-D(4) linker loops, resulted in, projection of the D(3) and D(4) in a spatially new orientation. We also, present a model for B(1)B(2)B(3)B(4). CONCLUSIONS: The B region of the Cna, collagen adhesin has a novel fold that is reminiscent of but is inverse in, nature to the IgG fold. This B region assembly could effectively provide, the needed flexibility and stability for presenting the ligand binding A, region away from the bacterial cell surface.
+
<StructureSection load='1d2p' size='340' side='right'caption='[[1d2p]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1d2p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D2P FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d2p OCA], [https://pdbe.org/1d2p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d2p RCSB], [https://www.ebi.ac.uk/pdbsum/1d2p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d2p ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CNA_STAAU CNA_STAAU] Mediates attachment of staphylococcal cells to collagen-containing substrata.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/1d2p_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d2p ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1D2P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D2P OCA].
+
*[[Adhesin 3D structures|Adhesin 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Novel fold and assembly of the repetitive B region of the Staphylococcus aureus collagen-binding surface protein., Deivanayagam CC, Rich RL, Carson M, Owens RT, Danthuluri S, Bice T, Hook M, Narayana SV, Structure. 2000 Jan 15;8(1):67-78. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10673425 10673425]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
-
[[Category: Deivanayagam, C.C.S.]]
+
[[Category: Deivanayagam CCS]]
-
[[Category: Hook, M.]]
+
[[Category: Hook M]]
-
[[Category: Narayana, S.V.L.]]
+
[[Category: Narayana SVL]]
-
[[Category: Rich, R.L.]]
+
[[Category: Rich RL]]
-
[[Category: cna]]
+
-
[[Category: collagen]]
+
-
[[Category: igg]]
+
-
[[Category: igsf]]
+
-
[[Category: mscramm]]
+
-
[[Category: staphylococcus aureus]]
+
-
[[Category: structural protein]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:58:25 2007''
+

Current revision

CRYSTAL STRUCTURE OF TWO B REPEAT UNITS (B1B2) OF THE COLLAGEN BINDING PROTEIN (CNA) OF STAPHYLOCOCCUS AUREUS

PDB ID 1d2p

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1d2p"
Personal tools