8pti

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(New page: 200px<br /><applet load="8pti" size="450" color="white" frame="true" align="right" spinBox="true" caption="8pti, resolution 1.8&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:8pti.gif|left|200px]]<br /><applet load="8pti" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="8pti, resolution 1.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN INHIBITOR'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN INHIBITOR==
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The structure of a Y35G mutant of bovine pancreatic trypsin inhibitor, (BPTI) was solved by molecular replacement and was refined by both, simulated annealing and restrained least-squares at 1.8 A resolution. The, crystals belong to the space group P42212, with unit cell dimensions a = b, = 46.75 A, c = 50.61 A. The final R-factor is 0.159 and the deviation from, ideality for bond distances is 0.02 A. The structure of the mutant differs, from that of the native protein, showing an overall root-mean-square, (r.m.s.) difference of 1.86 A for main-chain atoms. However, the change is, mostly localized in the two loops (respective r.m.s. values of 2.04 A and, 3.93 A) and the C terminus (r.m.s. 6.79 A), while the core of the protein, is well conserved (r.m.s. 0.45 A). The change in the loop regions can be, clearly attributed to the mutation while the difference in the C terminus, might be only due to a different crystal packing. Seventy water molecules, were included in the model but only seven of them are shared with the, native structure. Thermal parameters are showing a good correlation with, those for the wild-type of BPTI.
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<StructureSection load='8pti' size='340' side='right'caption='[[8pti]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8pti]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PTI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pti OCA], [https://pdbe.org/8pti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pti RCSB], [https://www.ebi.ac.uk/pdbsum/8pti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pti ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/8pti_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=8pti ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of a Y35G mutant of bovine pancreatic trypsin inhibitor (BPTI) was solved by molecular replacement and was refined by both simulated annealing and restrained least-squares at 1.8 A resolution. The crystals belong to the space group P42212, with unit cell dimensions a = b = 46.75 A, c = 50.61 A. The final R-factor is 0.159 and the deviation from ideality for bond distances is 0.02 A. The structure of the mutant differs from that of the native protein, showing an overall root-mean-square (r.m.s.) difference of 1.86 A for main-chain atoms. However, the change is mostly localized in the two loops (respective r.m.s. values of 2.04 A and 3.93 A) and the C terminus (r.m.s. 6.79 A), while the core of the protein is well conserved (r.m.s. 0.45 A). The change in the loop regions can be clearly attributed to the mutation while the difference in the C terminus might be only due to a different crystal packing. Seventy water molecules were included in the model but only seven of them are shared with the native structure. Thermal parameters are showing a good correlation with those for the wild-type of BPTI.
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==About this Structure==
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Crystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor.,Housset D, Kim KS, Fuchs J, Woodward C, Wlodawer A J Mol Biol. 1991 Aug 5;220(3):757-70. PMID:1714504<ref>PMID:1714504</ref>
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8PTI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=8PTI OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor., Housset D, Kim KS, Fuchs J, Woodward C, Wlodawer A, J Mol Biol. 1991 Aug 5;220(3):757-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1714504 1714504]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 8pti" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Fuchs, J.]]
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[[Category: Housset, D.]]
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[[Category: Kim, K.S.]]
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[[Category: Wlodawer, A.]]
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[[Category: Woodward, C.]]
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[[Category: proteinase inhibitor (trypsin)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:00:41 2007''
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==See Also==
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*[[BPTI 3D structures|BPTI 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Fuchs J]]
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[[Category: Housset D]]
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[[Category: Kim K-S]]
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[[Category: Wlodawer A]]
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[[Category: Woodward C]]

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CRYSTAL STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN INHIBITOR

PDB ID 8pti

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