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1dbg

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(New page: 200px<br /><applet load="1dbg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dbg, resolution 1.7&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1dbg.gif|left|200px]]<br /><applet load="1dbg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dbg, resolution 1.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CHONDROITINASE B'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CHONDROITINASE B==
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Glycosaminoglycans (GAGs) are a family of acidic heteropolysaccharides, including such molecules as chondroitin sulfate, dermatan sulfate, heparin, and keratan sulfate. Cleavage of the O-glycosidic bond within GAGs can be, accomplished by hydrolases as well as lyases, yielding disaccharide and, oligosaccharide products. We have determined the crystal structure of, chondroitinase B, a glycosaminoglycan lyase from Flavobacterium heparinum, as well as its complex with a dermatan sulfate disaccharide product, both, at 1.7 A resolution. Chondroitinase B adopts the right-handed parallel, beta-helix fold, found originally in pectate lyase and subsequently in, several polysaccharide lyases and hydrolases. Sequence homology between, chondroitinase B and a mannuronate lyase from Pseudomonas sp. suggests, this protein also adopts the beta-helix fold. Binding of the disaccharide, product occurs within a positively charged cleft formed by loops extending, from the surface of the beta-helix. Amino acid residues responsible for, recognition of the disaccharide, as well as potential catalytic residues, have been identified. Two arginine residues, Arg318 and Arg364, are found, to interact with the sulfate group attached to O-4 of, N-acetylgalactosamine. Cleavage of dermatan sulfate likely occurs at the, reducing end of the disaccharide, with Glu333 possibly acting as the, general base.
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<StructureSection load='1dbg' size='340' side='right'caption='[[1dbg]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1dbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pedobacter_heparinus Pedobacter heparinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBG FirstGlance]. <br>
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1DBG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pedobacter_heparinus Pedobacter heparinus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DBG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=G4D:4-DEOXY-ALPHA-D-GLUCOSE'>G4D</scene>, <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MXY:2-O-METHYL+FUCOSE'>MXY</scene>, <scene name='pdbligand=RAM:ALPHA-L-RHAMNOSE'>RAM</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbg OCA], [https://pdbe.org/1dbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbg RCSB], [https://www.ebi.ac.uk/pdbsum/1dbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbg ProSAT]</span></td></tr>
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Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution., Huang W, Matte A, Li Y, Kim YS, Linhardt RJ, Su H, Cygler M, J Mol Biol. 1999 Dec 17;294(5):1257-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10600383 10600383]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CSLB_PEDHD CSLB_PEDHD] Cleaves the glycosaminoglycan, dermatan sulfate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbg ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pedobacter heparinus]]
[[Category: Pedobacter heparinus]]
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[[Category: Single protein]]
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[[Category: Cygler M]]
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[[Category: Cygler, M.]]
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[[Category: Huang W]]
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[[Category: Huang, W.]]
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[[Category: Kim YS]]
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[[Category: Kim, Y.S.]]
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[[Category: Li Y]]
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[[Category: Li, Y.]]
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[[Category: Linhardt RJ]]
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[[Category: Linhardt, R.J.]]
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[[Category: Matte A]]
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[[Category: Matte, A.]]
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[[Category: Su H]]
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[[Category: Su, H.]]
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[[Category: beta helix]]
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[[Category: dematan sulfate]]
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[[Category: polysaccharide lyase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:08:19 2007''
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Current revision

CRYSTAL STRUCTURE OF CHONDROITINASE B

PDB ID 1dbg

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