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1dc7

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(New page: 200px<br /><applet load="1dc7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dc7" /> '''STRUCTURE OF A TRANSIENTLY PHOSPHORYLATED "S...)
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[[Image:1dc7.jpg|left|200px]]<br /><applet load="1dc7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dc7" />
 
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'''STRUCTURE OF A TRANSIENTLY PHOSPHORYLATED "SWITCH" IN BACTERIAL SIGNAL TRANSDUCTION'''<br />
 
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==Overview==
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==STRUCTURE OF A TRANSIENTLY PHOSPHORYLATED "SWITCH" IN BACTERIAL SIGNAL TRANSDUCTION==
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Receiver domains are the dominant molecular switches in bacterial, signalling. Although several structures of non-phosphorylated receiver, domains have been reported, a detailed structural understanding of the, activation arising from phosphorylation has been impeded by the very short, half-lives of the aspartylphosphate linkages. Here we present the first, structure of a receiver domain in its active state, the phosphorylated, receiver domain of the bacterial enhancer-binding protein NtrC (nitrogen, regulatory protein C). Nuclear magnetic resonance spectra were taken, during steady-state autophosphorylation/dephosphorylation, and, three-dimensional spectra from multiple samples were combined., Phosphorylation induces a large conformational change involving a, displacement of beta-strands 4 and 5 and alpha-helices 3 and 4 away from, the active site, a register shift and an axial rotation in helix 4. This, creates an exposed hydrophobic surface that is likely to transmit the, signal to the transcriptional activation domain.
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<StructureSection load='1dc7' size='340' side='right'caption='[[1dc7]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dc7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DC7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dc7 OCA], [https://pdbe.org/1dc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dc7 RCSB], [https://www.ebi.ac.uk/pdbsum/1dc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dc7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NTRC_SALTY NTRC_SALTY] Member of the two-component regulatory system NtrB/NtrC involved in the activation of nitrogen assimilatory genes such as GlnA. NtrC is phosphorylated by NtrB and interacts with sigma-54.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dc/1dc7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dc7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Receiver domains are the dominant molecular switches in bacterial signalling. Although several structures of non-phosphorylated receiver domains have been reported, a detailed structural understanding of the activation arising from phosphorylation has been impeded by the very short half-lives of the aspartylphosphate linkages. Here we present the first structure of a receiver domain in its active state, the phosphorylated receiver domain of the bacterial enhancer-binding protein NtrC (nitrogen regulatory protein C). Nuclear magnetic resonance spectra were taken during steady-state autophosphorylation/dephosphorylation, and three-dimensional spectra from multiple samples were combined. Phosphorylation induces a large conformational change involving a displacement of beta-strands 4 and 5 and alpha-helices 3 and 4 away from the active site, a register shift and an axial rotation in helix 4. This creates an exposed hydrophobic surface that is likely to transmit the signal to the transcriptional activation domain.
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==About this Structure==
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Structure of a transiently phosphorylated switch in bacterial signal transduction.,Kern D, Volkman BF, Luginbuhl P, Nohaile MJ, Kustu S, Wemmer DE Nature. 1999 Dec 23-30;402(6764):894-8. PMID:10622255<ref>PMID:10622255</ref>
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1DC7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DC7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of a transiently phosphorylated switch in bacterial signal transduction., Kern D, Volkman BF, Luginbuhl P, Nohaile MJ, Kustu S, Wemmer DE, Nature. 1999 Dec 23-30;402(6764):894-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10622255 10622255]
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</div>
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[[Category: Salmonella typhimurium]]
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<div class="pdbe-citations 1dc7" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Kern, D.]]
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<references/>
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[[Category: Kustu, S.]]
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__TOC__
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[[Category: Luginbuhl, P.]]
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</StructureSection>
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[[Category: Nohaile, M.J.]]
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[[Category: Large Structures]]
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[[Category: Volkman, B.F.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Wemmer, D.E.]]
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[[Category: Kern D]]
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[[Category: conformational rearrangement]]
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[[Category: Kustu S]]
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[[Category: phosphorylation]]
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[[Category: Luginbuhl P]]
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[[Category: receiver domain]]
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[[Category: Nohaile MJ]]
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[[Category: signal transduction]]
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[[Category: Volkman BF]]
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[[Category: two-component system]]
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[[Category: Wemmer DE]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:09:42 2007''
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Current revision

STRUCTURE OF A TRANSIENTLY PHOSPHORYLATED "SWITCH" IN BACTERIAL SIGNAL TRANSDUCTION

PDB ID 1dc7

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