1dhr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1dhr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dhr, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
Current revision (06:53, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1dhr.gif|left|200px]]<br /><applet load="1dhr" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1dhr, resolution 2.3&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE==
-
The structure of a binary complex of dihydropteridine reductase [DHPR;, NAD(P)H:6,7-dihydropteridine oxidoreductase, EC 1.6.99.7] with its, cofactor, NADH, has been solved and refined to a final R factor of 15.4%, by using 2.3 A diffraction data. DHPR is an alpha/beta protein with a, Rossmann-type dinucleotide fold for NADH binding. Insertion of an extra, threonine residue in the human enzyme is associated with severe symptoms, of a variant form of phenylketonuria and maps to a tightly linked sequence, of secondary-structural elements near the dimer interface. Dimerization is, mediated by a four-helix bundle motif (two helices from each protomer), having an unusual right-handed twist. DHPR is structurally and, mechanistically distinct from dihydrofolate reductase, appearing to more, closely resemble certain nicotinamide dinucleotide-requiring, flavin-dependent enzymes, such as glutathione reductase.
+
<StructureSection load='1dhr' size='340' side='right'caption='[[1dhr]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1dhr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The August 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Tetrahydrobiopterin Biosynthesis'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_8 10.2210/rcsb_pdb/mom_2015_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DHR FirstGlance]. <br>
-
1DHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DHR OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dhr OCA], [https://pdbe.org/1dhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dhr RCSB], [https://www.ebi.ac.uk/pdbsum/1dhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dhr ProSAT]</span></td></tr>
-
Crystal structure of rat liver dihydropteridine reductase., Varughese KI, Skinner MM, Whiteley JM, Matthews DA, Xuong NH, Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6080-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1631094 1631094]
+
</table>
-
[[Category: 6,7-dihydropteridine reductase]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/DHPR_RAT DHPR_RAT] The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dh/1dhr_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dhr ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
-
[[Category: Single protein]]
+
[[Category: Tetrahydrobiopterin Biosynthesis]]
-
[[Category: Matthews, D.A.]]
+
[[Category: Matthews DA]]
-
[[Category: Skinner, M.M.]]
+
[[Category: Skinner MM]]
-
[[Category: Varughese, K.I.]]
+
[[Category: Varughese KI]]
-
[[Category: Whiteley, J.M.]]
+
[[Category: Whiteley JM]]
-
[[Category: Xuong, N.H.]]
+
[[Category: Xuong NH]]
-
[[Category: NAD]]
+
-
[[Category: oxidoreductase(acting on nadh or nadph)]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:17:26 2007''
+

Current revision

CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE

PDB ID 1dhr

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1dhr"
Personal tools