1di0

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(New page: 200px<br /><applet load="1di0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1di0, resolution 2.7&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1di0.gif|left|200px]]<br /><applet load="1di0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1di0, resolution 2.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF LUMAZINE SYNTHASE FROM BRUCELLA ABORTUS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF LUMAZINE SYNTHASE FROM BRUCELLA ABORTUS==
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We have determined the three-dimensional structure of 6, 7-dimethyl-8-ribityllumazine synthase (lumazine synthase) from Brucella, abortus, the infectious organism of the disease brucellosis in animals., This enzyme catalyses the formation of 6, 7-dimethyl-8-ribityllumazine, the penultimate product in the synthesis of riboflavin. The, three-dimensional X-ray crystal structure of the enzyme from B. abortus, has been solved and refined at 2.7 A resolution to a final R-value of 0.18, (R(free)=0.23). The macromolecular assembly of the enzyme differs from, that of the enzyme from Bacillus subtilis, the only other lumazine, synthase structure known. While the protein from B. subtilis assembles, into a 60 subunit icosahedral capsid built from 12 pentameric units, the, enzyme from B. abortus is pentameric in its crystalline form. Nonetheless, the active sites of the two enzymes are virtually identical indicating, inhibitors to theses enzymes could be effective pharmaceuticals across a, broad species range. Furthermore, we compare the structures of the enzyme, from B. subtilis and B. abortus and describe the C teminus structure which, accounts for the differences in quaternary structure.
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<StructureSection load='1di0' size='340' side='right'caption='[[1di0]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1di0]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DI0 FirstGlance]. <br>
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1DI0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DI0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1di0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1di0 OCA], [https://pdbe.org/1di0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1di0 RCSB], [https://www.ebi.ac.uk/pdbsum/1di0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1di0 ProSAT]</span></td></tr>
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Divergence in macromolecular assembly: X-ray crystallographic structure analysis of lumazine synthase from Brucella abortus., Braden BC, Velikovsky CA, Cauerhff AA, Polikarpov I, Goldbaum FA, J Mol Biol. 2000 Apr 14;297(5):1031-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10764570 10764570]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RISB2_BRUAB RISB2_BRUAB] Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. Displays low catalytic activity in comparison with the isozyme RibH1.<ref>PMID:10482294</ref> <ref>PMID:16165152</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/di/1di0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1di0 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Brucella abortus]]
[[Category: Brucella abortus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Braden, B.C.]]
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[[Category: Braden BC]]
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[[Category: Cauerhff, A.A.]]
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[[Category: Cauerhff AA]]
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[[Category: Goldbaum, F.A.]]
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[[Category: Goldbaum FA]]
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[[Category: Polikarpov, I.]]
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[[Category: Polikarpov I]]
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[[Category: Velikovsky, C.A.]]
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[[Category: Velikovsky CA]]
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[[Category: PO4]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:17:34 2007''
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Current revision

CRYSTAL STRUCTURE OF LUMAZINE SYNTHASE FROM BRUCELLA ABORTUS

PDB ID 1di0

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