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1dl0

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(New page: 200px<br /><applet load="1dl0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dl0" /> '''SOLUTION STRUCTURE OF THE INSECTICIDAL NEURO...)
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[[Image:1dl0.gif|left|200px]]<br /><applet load="1dl0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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'''SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C==
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We have isolated a family of insect-selective neurotoxins from the venom, of the Australian funnel-web spider that appear to be good candidates for, biopesticide engineering. These peptides, which we have named the, Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with, four disulfide bridges, and they show no homology to any sequences in the, protein/DNA databases. The three-dimensional structure of one of these, toxins reveals an extremely rare vicinal disulfide bridge that we, demonstrate to be critical for insecticidal activity. We propose that, J-ACTX comprises an ancestral protein fold that we refer to as the, disulfide-directed beta-hairpin.
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<StructureSection load='1dl0' size='340' side='right'caption='[[1dl0]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dl0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DL0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dl0 OCA], [https://pdbe.org/1dl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dl0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dl0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TK1C_HADVE TK1C_HADVE] This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.<ref>PMID:10881200</ref> <ref>PMID:17141372</ref> <ref>PMID:18625007</ref> <ref>PMID:18986214</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.
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==About this Structure==
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Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge.,Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200<ref>PMID:10881200</ref>
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1DL0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge., Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF, Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10881200 10881200]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1dl0" style="background-color:#fffaf0;"></div>
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[[Category: King, G.F.]]
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== References ==
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[[Category: Wang, X.H.]]
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<references/>
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[[Category: atracotoxin]]
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__TOC__
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[[Category: cystine knot]]
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</StructureSection>
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[[Category: insecticidal]]
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[[Category: Hadronyche versuta]]
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[[Category: neurotoxin]]
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[[Category: Large Structures]]
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[[Category: vicinal disulfide]]
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[[Category: King GF]]
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[[Category: Wang XH]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:22:41 2007''
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Current revision

SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C

PDB ID 1dl0

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