1dl2

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(New page: 200px<br /><applet load="1dl2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dl2, resolution 1.54&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1dl2.gif|left|200px]]<br /><applet load="1dl2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dl2, resolution 1.54&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CLASS I ALPHA-1,2-MANNOSIDASE FROM SACCHAROMYCES CEREVISIAE AT 1.54 ANGSTROM RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CLASS I ALPHA-1,2-MANNOSIDASE FROM SACCHAROMYCES CEREVISIAE AT 1.54 ANGSTROM RESOLUTION==
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Mannose trimming is not only essential for N-glycan maturation in, mammalian cells but also triggers degradation of misfolded glycoproteins., The crystal structure of the class I alpha1, 2-mannosidase that trims, Man(9)GlcNAc(2) to Man(8)GlcNAc(2 )isomer B in the endoplasmic reticulum, of Saccharomyces cerevisiae reveals a novel (alphaalpha)(7)-barrel in, which an N-glycan from one molecule extends into the barrel of an adjacent, molecule, interacting with the essential acidic residues and calcium ion., The observed protein-carbohydrate interactions provide the first insight, into the catalytic mechanism and specificity of this eukaryotic enzyme, family and may be used to design inhibitors that prevent degradation of, misfolded glycoproteins in genetic diseases.
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<StructureSection load='1dl2' size='340' side='right'caption='[[1dl2]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dl2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DL2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dl2 OCA], [https://pdbe.org/1dl2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dl2 RCSB], [https://www.ebi.ac.uk/pdbsum/1dl2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dl2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MNS1_YEAST MNS1_YEAST] Involved in glycoprotein quality control as it is important for the targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations it further trims the carbohydrates to Man(5)GlcNAc(2).<ref>PMID:12090241</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dl/1dl2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dl2 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DL2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NAG, CA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_1,2-alpha-mannosidase Mannosyl-oligosaccharide 1,2-alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.113 3.2.1.113] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DL2 OCA].
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*[[Mannosidase 3D structures|Mannosidase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of a class I alpha1,2-mannosidase involved in N-glycan processing and endoplasmic reticulum quality control., Vallee F, Lipari F, Yip P, Sleno B, Herscovics A, Howell PL, EMBO J. 2000 Feb 15;19(4):581-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10675327 10675327]
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__TOC__
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[[Category: Mannosyl-oligosaccharide 1,2-alpha-mannosidase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Herscovics A]]
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[[Category: Herscovics, A.]]
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[[Category: Howell PL]]
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[[Category: Howell, P.L.]]
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[[Category: Lipari F]]
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[[Category: Lipari, F.]]
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[[Category: Vallee F]]
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[[Category: Vallee, F.]]
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[[Category: Yip P]]
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[[Category: Yip, P.]]
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[[Category: CA]]
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[[Category: GOL]]
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[[Category: NAG]]
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[[Category: alpha-alpha helix barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:22:45 2007''
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Current revision

CRYSTAL STRUCTURE OF CLASS I ALPHA-1,2-MANNOSIDASE FROM SACCHAROMYCES CEREVISIAE AT 1.54 ANGSTROM RESOLUTION

PDB ID 1dl2

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