1dla

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(New page: 200px<br /><applet load="1dla" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dla, resolution 3.0&Aring;" /> '''NOVEL NADPH-BINDING D...)
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[[Image:1dla.jpg|left|200px]]<br /><applet load="1dla" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dla, resolution 3.0&Aring;" />
 
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'''NOVEL NADPH-BINDING DOMAIN REVEALED BY THE CRYSTAL STRUCTURE OF ALDOSE REDUCTASE'''<br />
 
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==Overview==
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==NOVEL NADPH-BINDING DOMAIN REVEALED BY THE CRYSTAL STRUCTURE OF ALDOSE REDUCTASE==
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Aldose reductase is the first enzyme in the polyol pathway and catalyses, the NADPH-dependent reduction of D-glucose to D-sorbitol. Under normal, physiological conditions aldose reductase participates in osmoregulation, but under hyperglycaemic conditions it contributes to the onset and, development of severe complications in diabetes. Here we present the, crystal structure of pig lens aldose reductase refined to an R-factor of, 0.232 at 2.5-A resolution. It exhibits a single domain folded in an, eight-stranded parallel alpha/beta barrel, similar to that in triose, phosphate isomerase and a score of other enzymes. Hence, aldose reductase, does not possess the expected canonical dinucleotide-binding domain., Crystallographic analysis of the binding of, 2'-monophospho-adenosine-5'-diphosphoribose, which competitively inhibits, NADPH binding reveals that it binds into a cleft located at the C-terminal, end of the strands of the alpha/beta barrel. This represents a new type of, binding for nicotinamide adenine dinucleotide coenzymes.
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<StructureSection load='1dla' size='340' side='right'caption='[[1dla]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dla]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DLA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dla OCA], [https://pdbe.org/1dla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dla RCSB], [https://www.ebi.ac.uk/pdbsum/1dla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dla ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALDR_PIG ALDR_PIG] Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dl/1dla_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dla ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DLA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Active as [http://en.wikipedia.org/wiki/Aldehyde_reductase Aldehyde reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.21 1.1.1.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DLA OCA].
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*[[Aldose reductase 3D structures|Aldose reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Novel NADPH-binding domain revealed by the crystal structure of aldose reductase., Rondeau JM, Tete-Favier F, Podjarny A, Reymann JM, Barth P, Biellmann JF, Moras D, Nature. 1992 Jan 30;355(6359):469-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1734286 1734286]
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[[Category: Large Structures]]
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[[Category: Aldehyde reductase]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Barth, P.]]
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[[Category: Barth P]]
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[[Category: Biellmann, J.F.]]
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[[Category: Biellmann J-F]]
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[[Category: Moras, D.]]
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[[Category: Moras D]]
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[[Category: Podjarny, A.]]
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[[Category: Podjarny A]]
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[[Category: Reymann, J.M.]]
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[[Category: Reymann J-M]]
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[[Category: Rondeau, J.M.]]
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[[Category: Rondeau J-M]]
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[[Category: Tete-Favier, F.]]
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[[Category: Tete-Favier F]]
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[[Category: oxidoreductase(nadp)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:22:57 2007''
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Current revision

NOVEL NADPH-BINDING DOMAIN REVEALED BY THE CRYSTAL STRUCTURE OF ALDOSE REDUCTASE

PDB ID 1dla

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