1dm9

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(New page: 200px<br /><applet load="1dm9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dm9, resolution 2.0&Aring;" /> '''HEAT SHOCK PROTEIN 15...)
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[[Image:1dm9.gif|left|200px]]<br /><applet load="1dm9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dm9, resolution 2.0&Aring;" />
 
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'''HEAT SHOCK PROTEIN 15 KD'''<br />
 
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==Overview==
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==HEAT SHOCK PROTEIN 15 KD==
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We have solved the crystal structure of the heat shock protein Hsp15, a, newly isolated and very highly inducible heat shock protein that binds the, ribosome. Comparison of its structure with those of two RNA-binding, proteins, ribosomal protein S4 and threonyl-tRNA synthetase, reveals a, novel RNA-binding motif. This newly recognized motif is remarkably common, present in at least eight different protein families that bind RNA. The, motif's surface is populated by conserved, charged residues that define a, likely RNA-binding site. An intriguing pattern emerges: stress proteins, ribosomal proteins and tRNA synthetases repeatedly share a conserved, motif. This may imply a hitherto unrecognized functional similarity, between these three protein classes.
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<StructureSection load='1dm9' size='340' side='right'caption='[[1dm9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dm9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DM9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dm9 OCA], [https://pdbe.org/1dm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dm9 RCSB], [https://www.ebi.ac.uk/pdbsum/1dm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dm9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HSLR_ECOLI HSLR_ECOLI] Involved in the recycling of free 50S ribosomal subunits that still carry a nascent chain. Binds RNA more specifically than DNA. Binds with very high affinity to the free 50S ribosomal subunit. Does not bind it when it is part of the 70S ribosome.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dm/1dm9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dm9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DM9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DM9 OCA].
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*[[Heat Shock Protein structures|Heat Shock Protein structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of Hsp15 reveals a novel RNA-binding motif., Staker BL, Korber P, Bardwell JC, Saper MA, EMBO J. 2000 Feb 15;19(4):749-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10675344 10675344]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bardwell, J.C.A.]]
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[[Category: Bardwell JCA]]
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[[Category: Korber, P.]]
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[[Category: Korber P]]
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[[Category: Saper, M.A.]]
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[[Category: Saper MA]]
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[[Category: Staker, B.L.]]
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[[Category: Staker BL]]
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[[Category: SO4]]
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[[Category: heat shock proteins]]
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[[Category: protein-rna interactions]]
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[[Category: ribosome]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:24:12 2007''
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Current revision

HEAT SHOCK PROTEIN 15 KD

PDB ID 1dm9

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