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1h4g

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(New page: 200px<br /> <applet load="1h4g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h4g, resolution 1.10&Aring;" /> '''OLIGOSACCHARIDE-BIN...)
Current revision (11:27, 27 March 2024) (edit) (undo)
 
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[[Image:1h4g.gif|left|200px]]<br />
 
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<applet load="1h4g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h4g, resolution 1.10&Aring;" />
 
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'''OLIGOSACCHARIDE-BINDING TO FAMILY 11 XYLANASES: BOTH COVALENT INTERMEDIATE AND MUTANT-PRODUCT COMPLEXES DISPLAY 2,5B CONFORMATIONS AT THE ACTIVE-CENTRE'''<br />
 
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==Overview==
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==Oligosaccharide-binding to family 11 xylanases: both covalent intermediate and mutant-product complexes display 2,5B conformations at the active-centre==
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The glycoside hydrolase sequence-based classification reveals two families, of enzymes which hydrolyse the beta-1,4-linked backbone of xylan, xylanases, termed families GH-10 and GH-11. Family GH-11 xylanases are, intriguing in that catalysis is performed via a covalent intermediate, adopting an unusual (2,5)B (boat) conformation, a conformation which also, fulfils the stereochemical constraints of the oxocarbenium ion-like, transition state. Here, the 1.9 A structure of a nucleophile, E94A, mutant, of the Xyn11 from Bacillus agaradhaerens in complex with xylotriose is, presented. Intriguingly, this complex also adopts the (2,5)B conformation, in the -1 subsite, with the vacant space provided by the Glu--&gt;Ala, mutation allowing the sugar to adopt the alpha-configuration at C1. The, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11526340 (full description)]]
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<StructureSection load='1h4g' size='340' side='right'caption='[[1h4g]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h4g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salipaludibacillus_agaradhaerens Salipaludibacillus agaradhaerens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4G FirstGlance]. <br>
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1H4G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens]] with FXP and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H4G OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=X2F:2-DEOXY-2-FLUORO+XYLOPYRANOSE'>X2F</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4g OCA], [https://pdbe.org/1h4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4g RCSB], [https://www.ebi.ac.uk/pdbsum/1h4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4g ProSAT]</span></td></tr>
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Oligosaccharide binding to family 11 xylanases: both covalent intermediate and mutant product complexes display (2,5)B conformations at the active centre., Sabini E, Wilson KS, Danielsen S, Schulein M, Davies GJ, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1344-7. Epub 2001, Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11526340 11526340]
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</table>
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[[Category: Bacillus agaradhaerens]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q7SIE3_SALAG Q7SIE3_SALAG]
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[[Category: Danielsen, S.]]
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== Evolutionary Conservation ==
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[[Category: Davies, G.J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Sabini, E.]]
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Check<jmol>
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[[Category: Schulein, M.]]
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<jmolCheckbox>
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[[Category: Wilson, K.S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4g_consurf.spt"</scriptWhenChecked>
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[[Category: FXP]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: SO4]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: boat conformation]]
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</jmolCheckbox>
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[[Category: intermediate]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4g ConSurf].
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[[Category: mutant]]
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<div style="clear:both"></div>
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[[Category: oligosaccharide]]
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__TOC__
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[[Category: transition-state]]
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</StructureSection>
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[[Category: xylanase]]
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[[Category: Large Structures]]
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[[Category: Salipaludibacillus agaradhaerens]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:38:36 2007''
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[[Category: Danielsen S]]
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[[Category: Davies GJ]]
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[[Category: Sabini E]]
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[[Category: Schulein M]]
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[[Category: Wilson KS]]

Current revision

Oligosaccharide-binding to family 11 xylanases: both covalent intermediate and mutant-product complexes display 2,5B conformations at the active-centre

PDB ID 1h4g

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