1a3p

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{{Seed}}
 
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[[Image:1a3p.png|left|200px]]
 
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==ROLE OF THE 6-20 DISULFIDE BRIDGE IN THE STRUCTURE AND ACTIVITY OF EPIDERMAL GROWTH FACTOR, NMR, 20 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_1a3p", creates the "Structure Box" on the page.
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<StructureSection load='1a3p' size='340' side='right'caption='[[1a3p]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1a3p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A3P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABA:ALPHA-AMINOBUTYRIC+ACID'>ABA</scene></td></tr>
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{{STRUCTURE_1a3p| PDB=1a3p | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a3p OCA], [https://pdbe.org/1a3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a3p RCSB], [https://www.ebi.ac.uk/pdbsum/1a3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a3p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EGF_MOUSE EGF_MOUSE] EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/1a3p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a3p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two synthetic analogues of murine epidermal growth factor, [Abu6, 20] mEGF4-48 (where Abu denotes amino-butyric acid) and [G1, M3, K21, H40] mEGF1-48, have been investigated by NMR spectroscopy. [Abu6, 20] mEGF4-48 was designed to determine the contribution of the 6-20 disulfide bridge to the structure and function of mEGF. The overall structure of this analogue was similar to that of native mEGF, indicating that the loss of the 6-20 disulfide bridge did not affect the global fold of the molecule. Significant structural differences were observed near the N-terminus, however, with the direction of the polypeptide chain between residues four and nine being altered such that these residues were now located on the opposite face of the main beta-sheet from their position in native mEGF. Thermal denaturation experiments also showed that the structure of [Abu6, 20] mEGF4-48 was less stable than that of mEGF. Removal of this disulfide bridge resulted in a significant loss of both mitogenic activity in Balb/c 3T3 cells and receptor binding on A431 cells compared with native mEGF and mEGF4-48, implying that the structural changes in [Abu6, 20] mEGF4-48, although limited to the N-terminus, were sufficient to interfere with receptor binding. The loss of binding affinity probably arose mainly from steric interactions of the dislocated N-terminal region with part of the receptor binding surface of EGF. [G1, M3, K21, H40] mEGF1-48 was also synthesized in order to compare the synthetic polypeptide with the corresponding product of recombinant expression. Its mitogenic activity in Balb/c 3T3 cells was similar to that of native mEGF and analysis of its 1H chemical shifts suggested that its structure was also very similar to native.
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===ROLE OF THE 6-20 DISULFIDE BRIDGE IN THE STRUCTURE AND ACTIVITY OF EPIDERMAL GROWTH FACTOR, NMR, 20 STRUCTURES===
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Role of the 6-20 disulfide bridge in the structure and activity of epidermal growth factor.,Barnham KJ, Torres AM, Alewood D, Alewood PF, Domagala T, Nice EC, Norton RS Protein Sci. 1998 Aug;7(8):1738-49. PMID:10082370<ref>PMID:10082370</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1a3p" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10082370}}, adds the Publication Abstract to the page
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*[[Epidermal growth factor|Epidermal growth factor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10082370 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10082370}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1A3P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A3P OCA].
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==Reference==
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Role of the 6-20 disulfide bridge in the structure and activity of epidermal growth factor., Barnham KJ, Torres AM, Alewood D, Alewood PF, Domagala T, Nice EC, Norton RS, Protein Sci. 1998 Aug;7(8):1738-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10082370 10082370]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Alewood D]]
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[[Category: Alewood, D.]]
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[[Category: Alewood P]]
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[[Category: Alewood, P.]]
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[[Category: Barnham K]]
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[[Category: Barnham, K.]]
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[[Category: Domagala T]]
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[[Category: Domagala, T.]]
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[[Category: Nice E]]
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[[Category: Nice, E.]]
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[[Category: Norton R]]
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[[Category: Norton, R.]]
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[[Category: Torres A]]
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[[Category: Torres, A.]]
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[[Category: Disulfide connectivity]]
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[[Category: Egf-like domain]]
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[[Category: Growth factor]]
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[[Category: Murine epidermal growth factor]]
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[[Category: Repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 15:56:40 2008''
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Current revision

ROLE OF THE 6-20 DISULFIDE BRIDGE IN THE STRUCTURE AND ACTIVITY OF EPIDERMAL GROWTH FACTOR, NMR, 20 STRUCTURES

PDB ID 1a3p

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