1dqz

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(New page: 200px<br /><applet load="1dqz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dqz, resolution 1.50&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1dqz.gif|left|200px]]<br /><applet load="1dqz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dqz, resolution 1.50&Aring;" />
 
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'''CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS==
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The antigen 85 (ag85) complex, composed of three proteins (ag85A, B and, C), is a major protein component of the Mycobacterium tuberculosis cell, wall. Each protein possesses a mycolyltransferase activity required for, the biogenesis of trehalose dimycolate (cord factor), a dominant structure, necessary for maintaining cell wall integrity. The crystal structure of, recombinant ag85C from M. tuberculosis, refined to a resolution of 1.5 A, reveals an alpha/beta-hydrolase polypeptide fold, and a catalytic triad, formed by Ser 124, Glu 228 and His 260. ag85C complexed with a covalent, inhibitor implicates residues Leu 40 and Met 125 as components of the, oxyanion hole. A hydrophobic pocket and tunnel extending 21 A into the, core of the protein indicates the location of a probable trehalose, monomycolate binding site. Also, a large region of conserved surface, residues among ag85A, B and C is a probable site for the interaction of, ag85 proteins with human fibronectin.
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<StructureSection load='1dqz' size='340' side='right'caption='[[1dqz]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dqz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DQZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dqz OCA], [https://pdbe.org/1dqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dqz RCSB], [https://www.ebi.ac.uk/pdbsum/1dqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dqz ProSAT], [https://www.topsan.org/Proteins/TBSGC/1dqz TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A85C_MYCTU A85C_MYCTU] The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria to fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM.<ref>PMID:1830294</ref> <ref>PMID:9162010</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dq/1dqz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dqz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DQZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DQZ OCA].
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*[[Antigen 85 3D structures|Antigen 85 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of the secreted form of antigen 85C reveals potential targets for mycobacterial drugs and vaccines., Ronning DR, Klabunde T, Besra GS, Vissa VD, Belisle JT, Sacchettini JC, Nat Struct Biol. 2000 Feb;7(2):141-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10655617 10655617]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Klabunde T]]
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[[Category: Klabunde, T.]]
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[[Category: Ronning DR]]
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[[Category: Ronning, D.R.]]
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[[Category: Sacchettini JC]]
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[[Category: Sacchettini, J.C.]]
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[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
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[[Category: 85c]]
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[[Category: antigen]]
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[[Category: fibronectin]]
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[[Category: mycobacterium tuberculosis]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:30:39 2007''
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Current revision

CRYSTAL STRUCTURE OF ANTIGEN 85C FROM MYCOBACTERIUM TUBERCULOSIS

PDB ID 1dqz

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