1dtx

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(New page: 200px<br /><applet load="1dtx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dtx, resolution 2.2&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1dtx.gif|left|200px]]<br /><applet load="1dtx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dtx, resolution 2.2&Aring;" />
 
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'''CRYSTAL STRUCTURE OF ALPHA-DENDROTOXIN FROM THE GREEN MAMBA VENOM AND ITS COMPARISON WITH THE STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF ALPHA-DENDROTOXIN FROM THE GREEN MAMBA VENOM AND ITS COMPARISON WITH THE STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR==
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The three-dimensional structure of alpha-dendrotoxin (alpha-DTX) from the, green mamba (Dendroaspis angusticeps) venom has been determined, crystallographically using the method of isomorphous replacement and, refined at 2.2 A resolution using a restrained least-squares method. The, crystallographic R-factor is 0.169 for all 3451 measured reflections, between 7.0 and 2.2 A. Although the main-chain fold of alpha-DTX is, similar to that of homologous bovine pancreatic trypsin inhibitor (BPTI), there are significant differences involving segments of the polypeptide, chain close to the "antiprotease site" of BPTI. Comparison of the, structure of alpha-DTX with the existing models of BPTI and its complexes, with trypsin and kallikrein reveals structural differences that explain, the inability of alpha-DTX to inhibit trypsin and chymotrypsin.
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<StructureSection load='1dtx' size='340' side='right'caption='[[1dtx]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dtx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dendroaspis_angusticeps Dendroaspis angusticeps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DTX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dtx OCA], [https://pdbe.org/1dtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dtx RCSB], [https://www.ebi.ac.uk/pdbsum/1dtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dtx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VKTHA_DENAN VKTHA_DENAN] Serine protease inhibitor homolog that blocks voltage-gated potassium channels (Kv1.1/KCNA1, Kv1.2/KCNA2, and Kv1.6/KCNA6) (IC(50)=0.4-150 nM) and facilitates neurotransmitter release.<ref>PMID:10936620</ref> <ref>PMID:23771044</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/1dtx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dtx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DTX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dendroaspis_angusticeps Dendroaspis angusticeps] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DTX OCA].
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*[[Dendrotoxin|Dendrotoxin]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of alpha-dendrotoxin from the green mamba venom and its comparison with the structure of bovine pancreatic trypsin inhibitor., Skarzynski T, J Mol Biol. 1992 Apr 5;224(3):671-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1373774 1373774]
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__TOC__
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</StructureSection>
[[Category: Dendroaspis angusticeps]]
[[Category: Dendroaspis angusticeps]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Skarzynski, T.]]
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[[Category: Skarzynski T]]
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[[Category: SO4]]
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[[Category: presynaptic neurotoxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:34:37 2007''
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Current revision

CRYSTAL STRUCTURE OF ALPHA-DENDROTOXIN FROM THE GREEN MAMBA VENOM AND ITS COMPARISON WITH THE STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR

PDB ID 1dtx

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