1e14

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(New page: 200px<br /><applet load="1e14" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e14, resolution 2.7&Aring;" /> '''PHOTOSYNTHETIC REACTI...)
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[[Image:1e14.gif|left|200px]]<br /><applet load="1e14" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e14, resolution 2.7&Aring;" />
 
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'''PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M197 REPLACED WITH ARG (CHAIN M, FM197R) AND GLY M203 REPLACED WITH ASP (CHAIN M, GM203D)'''<br />
 
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==Overview==
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==PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M197 REPLACED WITH ARG (CHAIN M, FM197R) AND GLY M203 REPLACED WITH ASP (CHAIN M, GM203D)==
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Reaction centers with the double mutation Phe M197 to Arg and Gly M203 to, Asp (FM197R/GM203D) have been crystallized from an antenna-deficient, strain of Rhodobacter sphaeroides, and the structure has been determined, at 2.7 A resolution. Unlike in reaction centers with a single FM197R, mutation, the Arg M197 residue in the FM197R/GM203D reaction center adopts, a position similar to that of the native Phe residue in the wild-type, reaction center. Asp M203 is packed in such a way that the gamma-carboxy, group interacts with the backbone carbonyl of Arg M197. The Asp M203, residue takes up part of the volume that is occupied in the wild-type, reaction center by a water molecule. This water has been proposed to form, a hydrogen bond interaction with the 9-keto carbonyl group of the active, branch accessory bacteriochlorophyll, particularly when the primary donor, bacteriochlorophylls are oxidized. The GM203D mutation therefore appears, to remove the possibility of this hydrogen bond interaction by exclusion, of this water molecule, as well as altering the local dielectric, environment of the 9-keto carbonyl group. We examine whether the observed, structural changes can provide new or alternative explanations for the, absorbance and electron-transfer properties of reaction centers with the, FM197R and GM203D mutations.
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<StructureSection load='1e14' size='340' side='right'caption='[[1e14]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1e14]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E14 FirstGlance]. <br>
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1E14 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE, BCL, BPH, U10, SPN, LDA and CDL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E14 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e14 OCA], [https://pdbe.org/1e14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e14 RCSB], [https://www.ebi.ac.uk/pdbsum/1e14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e14 ProSAT]</span></td></tr>
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Structural consequences of the replacement of glycine M203 with aspartic acid in the reaction center from Rhodobacter sphaeroides., Fyfe PK, Ridge JP, McAuley KE, Cogdell RJ, Isaacs NW, Jones MR, Biochemistry. 2000 May 23;39(20):5953-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10821666 10821666]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Rhodobacter sphaeroides]]
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[https://www.uniprot.org/uniprot/RCEH_CERSP RCEH_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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[[Category: Cogdell, R.J.]]
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== Evolutionary Conservation ==
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[[Category: Fyfe, P.K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Isaacs, N.W.]]
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Check<jmol>
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[[Category: Jones, M.R.]]
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<jmolCheckbox>
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[[Category: Mcauley, K.E.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e1/1e14_consurf.spt"</scriptWhenChecked>
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[[Category: Ridge, J.P.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: BCL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: BPH]]
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</jmolCheckbox>
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[[Category: CDL]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e14 ConSurf].
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[[Category: FE]]
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<div style="clear:both"></div>
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[[Category: LDA]]
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__TOC__
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[[Category: SPN]]
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</StructureSection>
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[[Category: U10]]
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[[Category: Cereibacter sphaeroides]]
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[[Category: electron transport]]
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[[Category: Large Structures]]
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[[Category: photosynthesis]]
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[[Category: Cogdell RJ]]
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[[Category: photosynthetic reaction center]]
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[[Category: Fyfe PK]]
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[[Category: transmembrane]]
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[[Category: Isaacs NW]]
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[[Category: Jones MR]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:43:28 2007''
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[[Category: McAuley KE]]
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[[Category: Ridge JP]]

Current revision

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH PHE M197 REPLACED WITH ARG (CHAIN M, FM197R) AND GLY M203 REPLACED WITH ASP (CHAIN M, GM203D)

PDB ID 1e14

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