1b2r

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{{Seed}}
 
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[[Image:1b2r.png|left|200px]]
 
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==FERREDOXIN-NADP+ REDUCTASE (MUTATION: E 301 A)==
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The line below this paragraph, containing "STRUCTURE_1b2r", creates the "Structure Box" on the page.
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<StructureSection load='1b2r' size='340' side='right'caption='[[1b2r]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1b2r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7119 Nostoc sp. PCC 7119]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B2R FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1b2r| PDB=1b2r | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b2r OCA], [https://pdbe.org/1b2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b2r RCSB], [https://www.ebi.ac.uk/pdbsum/1b2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b2r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FENR_NOSSO FENR_NOSSO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/1b2r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b2r ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional crystal structure of the Glu301Ala site-directed mutant of ferredoxin-NADP+ reductase from Anabaena PCC 7119 has been determined at 1.8A resolution by x-ray diffraction. The overall folding of the Glu301Ala FNR mutant shows no significant differences with respect to that of the wild-type enzyme. However, interesting conformational changes are detected in the side chain of another glutamate residue, Glu139, which now points towards the FAD cofactor in the active center cavity. The new conformation of the Glu139 side chain is stabilized by a network of five hydrogen bonds to several water molecules, which seem to hold the carboxylate side chain in a rather fixed position. This interacting network connects the Glu139 side chain to the Ser80 side chain through a series of three water molecules. These observations are discussed in terms of the reactivity of Glu301Ala ferredoxin-NADP+ reductase towards its substrates, and the role of Glu301 in the catalysis is re-examined. Moreover, a structural explanation of the different reoxidation properties of this mutant is given on the basis of the reported structure by modeling the hypothetical flavin C(4a)-hydroperoxide intermediate. The model shows that the distal oxygen of the peroxide anion could be in an appropriate situation to act as the proton donor in the reoxidation process.
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===FERREDOXIN-NADP+ REDUCTASE (MUTATION: E 301 A)===
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Structural basis of the catalytic role of Glu301 in Anabaena PCC 7119 ferredoxin-NADP+ reductase revealed by x-ray crystallography.,Mayoral T, Medina M, Sanz-Aparicio J, Gomez-Moreno C, Hermoso JA Proteins. 2000 Jan 1;38(1):60-9. PMID:10651039<ref>PMID:10651039</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10651039}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1b2r" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10651039 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10651039}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1B2R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2R OCA].
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[[Category: Nostoc sp. PCC 7119]]
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[[Category: Gomez-Moreno C]]
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==Reference==
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[[Category: Hermoso JA]]
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Structural basis of the catalytic role of Glu301 in Anabaena PCC 7119 ferredoxin-NADP+ reductase revealed by x-ray crystallography., Mayoral T, Medina M, Sanz-Aparicio J, Gomez-Moreno C, Hermoso JA, Proteins. 2000 Jan 1;38(1):60-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10651039 10651039]
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[[Category: Martinez-Julvez M]]
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[[Category: Anabaena sp.]]
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[[Category: Martinez-Ripoll M]]
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[[Category: Single protein]]
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[[Category: Mayoral T]]
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[[Category: Gomez-Moreno, C.]]
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[[Category: Medina M]]
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[[Category: Hermoso, J A.]]
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[[Category: Sanz-Aparicio J]]
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[[Category: Martinez-Julvez, M.]]
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[[Category: Martinez-Ripoll, M.]]
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[[Category: Mayoral, T.]]
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[[Category: Medina, M.]]
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[[Category: Sanz-Aparicio, J.]]
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[[Category: Fad]]
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[[Category: Flavoprotein]]
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[[Category: Fnr]]
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[[Category: Nadp]]
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[[Category: Nadp reductase]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:05:57 2008''
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Current revision

FERREDOXIN-NADP+ REDUCTASE (MUTATION: E 301 A)

PDB ID 1b2r

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