This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1ebf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1ebf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ebf, resolution 2.30&Aring;" /> '''HOMOSERINE DEHYDROGE...)
Current revision (07:00, 7 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ebf.jpg|left|200px]]<br /><applet load="1ebf" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ebf, resolution 2.30&Aring;" />
 
-
'''HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+'''<br />
 
-
==Overview==
+
==HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+==
-
The structure of the antifungal drug target homoserine dehydrogenase (HSD), was determined from Saccharomyces cerevisiae in apo and holo forms, and as, a ternary complex with bound products, by X-ray diffraction. The three, forms show that the enzyme is a dimer, with each monomer composed of three, regions, the nucleotide-binding region, the dimerization region and the, catalytic region. The dimerization and catalytic regions have novel folds, whereas the fold of the nucleotide-binding region is a variation on the, Rossmann fold. The novel folds impose a novel composition and arrangement, of active site residues when compared to all other currently known, oxidoreductases. This observation, in conjunction with site-directed, mutagenesis of active site residues and steady-state kinetic measurements, suggest that HSD exhibits a new variation on dehydrogenase chemistry.
+
<StructureSection load='1ebf' size='340' side='right'caption='[[1ebf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1ebf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EBF FirstGlance]. <br>
-
1EBF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with NA and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Homoserine_dehydrogenase Homoserine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.3 1.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EBF OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ebf OCA], [https://pdbe.org/1ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ebf RCSB], [https://www.ebi.ac.uk/pdbsum/1ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ebf ProSAT]</span></td></tr>
-
Crystal structures of homoserine dehydrogenase suggest a novel catalytic mechanism for oxidoreductases., DeLaBarre B, Thompson PR, Wright GD, Berghuis AM, Nat Struct Biol. 2000 Mar;7(3):238-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10700284 10700284]
+
</table>
-
[[Category: Homoserine dehydrogenase]]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/DHOM_YEAST DHOM_YEAST]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/1ebf_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ebf ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Single protein]]
+
[[Category: Berghuis AM]]
-
[[Category: Berghuis, A.M.]]
+
[[Category: DeLaBarre B]]
-
[[Category: DeLaBarre, B.]]
+
[[Category: Thompson PR]]
-
[[Category: Thompson, P.R.]]
+
[[Category: Wright GD]]
-
[[Category: Wright, G.D.]]
+
-
[[Category: NA]]
+
-
[[Category: NAD]]
+
-
[[Category: dehydrogenase]]
+
-
[[Category: dimer]]
+
-
[[Category: dinucleotide]]
+
-
[[Category: homoserine]]
+
-
[[Category: nad]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:52:31 2007''
+

Current revision

HOMOSERINE DEHYDROGENASE FROM S. CEREVISIAE COMPLEX WITH NAD+

PDB ID 1ebf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ebf"
Personal tools