1ecs

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(New page: 200px<br /><applet load="1ecs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ecs, resolution 1.70&Aring;" /> '''THE 1.7 A CRYSTAL ST...)
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[[Image:1ecs.jpg|left|200px]]<br /><applet load="1ecs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ecs, resolution 1.70&Aring;" />
 
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'''THE 1.7 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT ENCODED ON THE TRANSPOSON TN5'''<br />
 
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==Overview==
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==THE 1.7 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT ENCODED ON THE TRANSPOSON TN5==
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The transposon Tn5 carries a gene designated ble that confers resistance, to bleomycin (Bm). In this study, we determined the x-ray crystal, structures of the ble gene product, designated BLMT, uncomplexed and, complexed with Bm at 1.7 and 2.5 A resolution, respectively. The structure, of BLMT is a dimer with two Bm-binding pockets composed of two large, concavities and two long grooves. This crystal structure of BLMT complexed, with Bm gives a precise mode for binding of the antibiotic to BLMT. The, conformational change of BLMT generated by binding to Bm occurs at a, beta-turn composed of the residues from Gln(97) to Thr(102)., Crystallographic analysis of Bm bound to BLMT shows that two thiazolium, rings of the bithiazole moiety are in the trans conformation. The axial, ligand, which binds a metal ion, seems to be the primary amine in the, beta-aminoalanine moiety. This report, which is the first with regard to, the x-ray crystal structure of Bm, shows that the bithiazole moiety of Bm, is far from the metal-binding domain. That is, Bm complexed with BLMT, takes a more extended form than the drug complexed with DNA.
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<StructureSection load='1ecs' size='340' side='right'caption='[[1ecs]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ecs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ECS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ecs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ecs OCA], [https://pdbe.org/1ecs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ecs RCSB], [https://www.ebi.ac.uk/pdbsum/1ecs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ecs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLE_KLEPN BLE_KLEPN] Binding protein with a strong affinity to the bleomycin family of antibiotics. Binds to CL990; an antimitotic-antibiotic compound.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/1ecs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ecs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The transposon Tn5 carries a gene designated ble that confers resistance to bleomycin (Bm). In this study, we determined the x-ray crystal structures of the ble gene product, designated BLMT, uncomplexed and complexed with Bm at 1.7 and 2.5 A resolution, respectively. The structure of BLMT is a dimer with two Bm-binding pockets composed of two large concavities and two long grooves. This crystal structure of BLMT complexed with Bm gives a precise mode for binding of the antibiotic to BLMT. The conformational change of BLMT generated by binding to Bm occurs at a beta-turn composed of the residues from Gln(97) to Thr(102). Crystallographic analysis of Bm bound to BLMT shows that two thiazolium rings of the bithiazole moiety are in the trans conformation. The axial ligand, which binds a metal ion, seems to be the primary amine in the beta-aminoalanine moiety. This report, which is the first with regard to the x-ray crystal structure of Bm, shows that the bithiazole moiety of Bm is far from the metal-binding domain. That is, Bm complexed with BLMT takes a more extended form than the drug complexed with DNA.
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==About this Structure==
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Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin.,Maruyama M, Kumagai T, Matoba Y, Hayashida M, Fujii T, Hata Y, Sugiyama M J Biol Chem. 2001 Mar 30;276(13):9992-9. Epub 2000 Dec 29. PMID:11134052<ref>PMID:11134052</ref>
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1ECS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with CA and PG4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ECS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin., Maruyama M, Kumagai T, Matoba Y, Hayashida M, Fujii T, Hata Y, Sugiyama M, J Biol Chem. 2001 Mar 30;276(13):9992-9. Epub 2000 Dec 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11134052 11134052]
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</div>
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<div class="pdbe-citations 1ecs" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kumagai, T.]]
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[[Category: Kumagai T]]
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[[Category: Maruyama, M.]]
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[[Category: Maruyama M]]
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[[Category: Matoba, Y.]]
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[[Category: Matoba Y]]
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[[Category: Sugiyama, M.]]
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[[Category: Sugiyama M]]
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[[Category: CA]]
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[[Category: PG4]]
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[[Category: arm-exchange]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:54:14 2007''
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Current revision

THE 1.7 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT ENCODED ON THE TRANSPOSON TN5

PDB ID 1ecs

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