1bhr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:36, 22 November 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1bhr.png|left|200px]]
 
-
<!--
+
==2'-DEOXY-ISOGUANOSINE BASE PAIRED TO THYMIDINE, NMR, MINIMIZED AVERAGE STRUCTURE==
-
The line below this paragraph, containing "STRUCTURE_1bhr", creates the "Structure Box" on the page.
+
<StructureSection load='1bhr' size='340' side='right'caption='[[1bhr]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1bhr]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BHR FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IGU:2-DEOXYISOGUANINE-5-MONOPHOSPHATE'>IGU</scene></td></tr>
-
{{STRUCTURE_1bhr| PDB=1bhr | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhr OCA], [https://pdbe.org/1bhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bhr RCSB], [https://www.ebi.ac.uk/pdbsum/1bhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bhr ProSAT]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The questions of whether different tautomeric forms of nucleic acid bases exist to any significant extent in DNA, or what their possible roles in mutation may be, are under intense scrutiny. 2'-Deoxyisoguanosine (iG) has been suggested to have a propensity to adopt the enol form. Isoguanine (also called 2-hydroxyadenine) can be found in oxidatively damaged DNA generated from treating DNA with a Fenton-type reactive oxygen-generating system and is known to cause mutation. We have analyzed the three-dimensional structure of the DNA dodecamer d(CGC[iG]AATTTGCG) (denoted iG-DODE) by X-ray crystallography and NMR. The crystal structure of the iG-DODE complexed with the minor groove binder Hoechst 33342, refined to 1.4 A resolution, showed that the two independent iG.T base pairs in the dodecamer duplex adopt different (one in Watson-Crick and the other in wobble) conformations. The high-resolution nature of the structure also affords unprecedented clear information about the conformation and interactions of the Hoechst drug. The Hoechst 33342 binds in the narrow minor groove at the iGAATT site, with the N-methylpiperazine ring near the iG4.T21 base pair. Three hydrogen bonds are found between the NH of the Hoechst ligand and T-O2 DNA atoms. In solution, the two iG.T base pairs in iG-DODE predominantly are in the wobble form at 2 degreesC. At higher temperatures, another duplex form (likely involving the enol form of iG) is in slow exchange with the keto form and becomes significantly populated, reaching approximately 40% at 40 degreesC. Our data support the conclusion that iG pairs with T in a Watson-Crick configuration to a significant extent at physiological temperature (37 degreesC), which may explain the facile incorporation rate of T across from an iG during in vitro DNA replication.
-
===2'-DEOXY-ISOGUANOSINE BASE PAIRED TO THYMIDINE, NMR, MINIMIZED AVERAGE STRUCTURE===
+
2'-Deoxyisoguanosine adopts more than one tautomer to form base pairs with thymidine observed by high-resolution crystal structure analysis.,Robinson H, Gao YG, Bauer C, Roberts C, Switzer C, Wang AH Biochemistry. 1998 Aug 4;37(31):10897-905. PMID:9692982<ref>PMID:9692982</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_9692982}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1bhr" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 9692982 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_9692982}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHR OCA].
+
[[Category: Bauer C]]
-
 
+
[[Category: Gao Y-G]]
-
==Reference==
+
[[Category: Roberts C]]
-
2'-Deoxyisoguanosine adopts more than one tautomer to form base pairs with thymidine observed by high-resolution crystal structure analysis., Robinson H, Gao YG, Bauer C, Roberts C, Switzer C, Wang AH, Biochemistry. 1998 Aug 4;37(31):10897-905. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9692982 9692982]
+
[[Category: Robinson H]]
-
[[Category: Bauer, C.]]
+
[[Category: Switzer C]]
-
[[Category: Gao, Y G.]]
+
[[Category: Wang AH-J]]
-
[[Category: Roberts, C.]]
+
-
[[Category: Robinson, H.]]
+
-
[[Category: Switzer, C.]]
+
-
[[Category: Wang, A H.J.]]
+
-
[[Category: Deoxyribonucleic acid]]
+
-
[[Category: Isoguanosine-thymidine mismatch]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:11:15 2008''
+

Current revision

2'-DEOXY-ISOGUANOSINE BASE PAIRED TO THYMIDINE, NMR, MINIMIZED AVERAGE STRUCTURE

PDB ID 1bhr

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bhr"
Personal tools