1egp

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(New page: 200px<br /><applet load="1egp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1egp, resolution 2.0&Aring;" /> '''PROTEINASE INHIBITOR ...)
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[[Image:1egp.jpg|left|200px]]<br /><applet load="1egp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1egp, resolution 2.0&Aring;" />
 
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'''PROTEINASE INHIBITOR EGLIN C WITH HYDROLYSED REACTIVE CENTER'''<br />
 
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==Overview==
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==PROTEINASE INHIBITOR EGLIN C WITH HYDROLYSED REACTIVE CENTER==
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The inhibition of serine proteinases by both synthetic and natural, inhibitors has been widely studied. Eglin c is a small thermostable, protein isolated from the leech, Hirudo medicinalis. Eglin c is a potent, serine proteinase inhibitor. The three-dimensional structure of native, eglin and of its complexes with a number of proteinases are known. We here, describe the crystal structure of hydrolysed eglin not bound to a, proteinase. The body of the eglin has a conformation remarkably similar to, that in the known complexes with proteinases. However, the peptide chain, has been cut at the 'scissile' bond between residues 45 and 46, presumed, to result from the presence of subtilisin DY in the crystallisation, sample. The residues usually making up the inhibiting loop of eglin take, up a quite different conformation in the nicked inhibitor leading to, stabilising contacts between neighbouring molecules in the crystal. The, structure was solved by molecular replacement techniques and refined to a, final R-factor of 14.5%.
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<StructureSection load='1egp' size='340' side='right'caption='[[1egp]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1egp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EGP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1egp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1egp OCA], [https://pdbe.org/1egp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1egp RCSB], [https://www.ebi.ac.uk/pdbsum/1egp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1egp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ICIC_HIRME ICIC_HIRME] Inhibits both elastase and cathepsin G.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1egp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1egp ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EGP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EGP OCA].
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*[[Eglin|Eglin]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the proteinase inhibitor eglin c with hydrolysed reactive centre at 2.0 A resolution., Betzel C, Dauter Z, Genov N, Lamzin V, Navaza J, Schnebli HP, Visanji M, Wilson KS, FEBS Lett. 1993 Feb 15;317(3):185-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8425603 8425603]
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[[Category: Hirudo medicinalis]]
[[Category: Hirudo medicinalis]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Betzel, C.]]
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[[Category: Betzel C]]
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[[Category: Dauter, Z.]]
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[[Category: Dauter Z]]
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[[Category: Lamzin, V.]]
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[[Category: Lamzin V]]
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[[Category: Wilson, K.S.]]
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[[Category: Wilson KS]]
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[[Category: proteinase inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:59:31 2007''
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Current revision

PROTEINASE INHIBITOR EGLIN C WITH HYDROLYSED REACTIVE CENTER

PDB ID 1egp

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