1egu

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1egu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1egu, resolution 1.56&Aring;" /> '''CRYSTAL STRUCTURE OF...)
Current revision (07:02, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1egu.gif|left|200px]]<br /><applet load="1egu" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1egu, resolution 1.56&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE AT 1.56 A RESOLUTION'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE AT 1.56 A RESOLUTION==
-
Streptococcus pneumoniae hyaluronate lyase (spnHL) is a pathogenic, bacterial spreading factor and cleaves hyaluronan, an important, constituent of the extra- cellular matrix of connective tissues, through, an enzymatic beta-elimination process, different from the hyaluronan, degradation by hydrolases in animals. The mechanism of hyaluronan binding, and degradation was proposed based on the 1.56 A resolution crystal, structure, substrate modeling and mutagenesis studies on spnHL. Five, mutants, R243V, N349A, H399A, Y408F and N580G, were constructed and their, activities confirmed our mechanism hypothesis. The important roles of, Tyr408, Asn349 and His399 in enzyme catalysis were proposed, explained and, confirmed by mutant studies. The remaining weak enzymatic activity of the, H399A mutant, the role of the free carboxylate group on the glucuronate, residue, the enzymatic behavior on chondroitin and chondroitin sulfate, and the small activity increase in the N580G mutant were explained based, on this mechanism. A possible function of the C-terminal beta-sheet domain, is to modulate enzyme activity through binding to calcium ions.
+
<StructureSection load='1egu' size='340' side='right'caption='[[1egu]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1egu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EGU FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1egu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1egu OCA], [https://pdbe.org/1egu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1egu RCSB], [https://www.ebi.ac.uk/pdbsum/1egu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1egu ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/HYSA_STRPN HYSA_STRPN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1egu_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1egu ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1EGU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Hyaluronate_lyase Hyaluronate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.1 4.2.2.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EGU OCA].
+
*[[Hyaluronidase 3D structures|Hyaluronidase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structural basis of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase., Li S, Kelly SJ, Lamani E, Ferraroni M, Jedrzejas MJ, EMBO J. 2000 Mar 15;19(6):1228-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10716923 10716923]
+
[[Category: Large Structures]]
-
[[Category: Hyaluronate lyase]]
+
-
[[Category: Single protein]]
+
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
-
[[Category: Ferraroni, M.]]
+
[[Category: Ferraroni M]]
-
[[Category: Jedrzejas, M.J.]]
+
[[Category: Jedrzejas MJ]]
-
[[Category: Kelly, S.J.]]
+
[[Category: Kelly SJ]]
-
[[Category: Lamani, E.]]
+
[[Category: Lamani E]]
-
[[Category: Li, S.]]
+
[[Category: Li S]]
-
[[Category: SO4]]
+
-
[[Category: (alfa5/alfa5) barrel]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:59:50 2007''
+

Current revision

CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE AT 1.56 A RESOLUTION

PDB ID 1egu

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1egu"
Personal tools