1bry

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{{Seed}}
 
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[[Image:1bry.png|left|200px]]
 
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==BRYODIN TYPE I RIP==
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The line below this paragraph, containing "STRUCTURE_1bry", creates the "Structure Box" on the page.
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<StructureSection load='1bry' size='340' side='right'caption='[[1bry]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1bry]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bryonia_dioica Bryonia dioica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BRY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bry OCA], [https://pdbe.org/1bry PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bry RCSB], [https://www.ebi.ac.uk/pdbsum/1bry PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bry ProSAT]</span></td></tr>
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{{STRUCTURE_1bry| PDB=1bry | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIP1_BRYDI RIP1_BRYDI] Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/1bry_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bry ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bryonia dioica (Cucurbitaceae family) produces at least two type I ribosome-inactivating proteins, bryodin 1 (BD1) and bryodin 2 (BD2). A cDNA sequence encoding BD1 was isolated from B. dioica leaf mRNA using degenerative oligonucleotides and codes for a 22 amino acid signal peptide followed by a protein of 267 residues. Expression of two recombinant BD1 (rBD1) forms in Escherichia coli yielded proteins of 267 (to the natural stop codon) and 247 amino acids (to the putative cleavage site yielding the mature protein) that had identical protein synthesis inhibition activity as compared to native BD1. The substitution of Lys for Glu at position 189 near the active site reduced the ability of rBD1 to inhibit protein synthesis by 10-fold. Toxicologic analysis showed that rBD1 was well tolerated in rodents with LD50 values of 40 mg/kg in mice and &gt;25 mg/kg in rats. A crystal of mature rBD1 protein was used to collect X-ray diffraction data to 2.1 A resolution. The rBD1 crystal structure was solved and showed extensive homology with other type I RIPs and A chains of type II RIPs. The studies described here demonstrate that rBD1 retains full biologic activity and serve as a guide for using this potent, yet nontoxic, RIP in the construction of single-chain immunotoxin fusion proteins.
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===BRYODIN TYPE I RIP===
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Molecular, biological, and preliminary structural analysis of recombinant bryodin 1, a ribosome-inactivating protein from the plant Bryonia dioica.,Gawlak SL, Neubauer M, Klei HE, Chang CY, Einspahr HM, Siegall CB Biochemistry. 1997 Mar 18;36(11):3095-103. PMID:9115985<ref>PMID:9115985</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_9115985}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1bry" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 9115985 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9115985}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1BRY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bryonia_dioica Bryonia dioica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BRY OCA].
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==Reference==
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Molecular, biological, and preliminary structural analysis of recombinant bryodin 1, a ribosome-inactivating protein from the plant Bryonia dioica., Gawlak SL, Neubauer M, Klei HE, Chang CY, Einspahr HM, Siegall CB, Biochemistry. 1997 Mar 18;36(11):3095-103. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9115985 9115985]
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[[Category: Bryonia dioica]]
[[Category: Bryonia dioica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: RRNA N-glycosylase]]
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[[Category: Chang CY]]
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[[Category: Chang, C Y.]]
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[[Category: Klei HE]]
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[[Category: Klei, H E.]]
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[[Category: Bryodin]]
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[[Category: Immunotoxin]]
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[[Category: Ribosome-inactivating protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:37:47 2008''
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Current revision

BRYODIN TYPE I RIP

PDB ID 1bry

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