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1ei9

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(New page: 200px<br /><applet load="1ei9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ei9, resolution 2.25&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1ei9.jpg|left|200px]]<br /><applet load="1ei9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ei9, resolution 2.25&Aring;" />
 
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'''CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE 1'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE 1==
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Mutations in palmitoyl-protein thioesterase 1 (PPT1), a lysosomal enzyme, that removes fatty acyl groups from cysteine residues in modified, proteins, cause the fatal inherited neurodegenerative disorder infantile, neuronal ceroid lipofuscinosis. The accumulation of undigested substrates, leads to the formation of neuronal storage bodies that are associated with, the clinical symptoms. Less severe forms of PPT1 deficiency have been, found recently that are caused by a distinct set of PPT1 mutations, some, of which retain a small amount of thioesterase activity. We have, determined the crystal structure of PPT1 with and without bound palmitate, by using multiwavelength anomalous diffraction phasing. The structure, reveals an alpha/beta-hydrolase fold with a catalytic triad composed of, Ser115-His289-Asp233 and provides insights into the structural basis for, the phenotypes associated with PPT1 mutations.
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<StructureSection load='1ei9' size='340' side='right'caption='[[1ei9]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ei9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EI9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ei9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ei9 OCA], [https://pdbe.org/1ei9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ei9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ei9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ei9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPT1_BOVIN PPT1_BOVIN] Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1ei9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ei9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EI9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with NDG and NAG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Palmitoyl-protein_hydrolase Palmitoyl-protein hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.22 3.1.2.22] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EI9 OCA].
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*[[Thioesterase 3D structures|Thioesterase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of palmitoyl protein thioesterase 1 and the molecular basis of infantile neuronal ceroid lipofuscinosis., Bellizzi JJ 3rd, Widom J, Kemp C, Lu JY, Das AK, Hofmann SL, Clardy J, Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4573-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10781062 10781062]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Palmitoyl-protein hydrolase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Bellizzi III JJ]]
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[[Category: Clardy, J.]]
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[[Category: Clardy J]]
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[[Category: Das, A.K.]]
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[[Category: Das AK]]
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[[Category: Hofmann, S.L.]]
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[[Category: Hofmann SL]]
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[[Category: III, J.J.Bellizzi.]]
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[[Category: Kemp C]]
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[[Category: Kemp, C.]]
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[[Category: Lu JY]]
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[[Category: Lu, J.Y.]]
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[[Category: Widom J]]
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[[Category: Widom, J.]]
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[[Category: NAG]]
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[[Category: NDG]]
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[[Category: alpha/beta hydrolase]]
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[[Category: glycoprotein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:01:08 2007''
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Current revision

CRYSTAL STRUCTURE OF PALMITOYL PROTEIN THIOESTERASE 1

PDB ID 1ei9

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