1ek8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1ek8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ek8, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
Current revision (07:03, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ek8.gif|left|200px]]<br /><applet load="1ek8" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ek8, resolution 2.3&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI==
-
We have determined the crystal structure of the Escherichia coli ribosome, recycling factor (RRF), which catalyzes the disassembly of the termination, complex in protein synthesis. The L-shaped molecule consists of two, domains: a triple-stranded antiparallel coiled-coil and an alpha/beta, domain. The coil domain has a cylindrical shape and negatively charged, surface, which are reminiscent of the anticodon arm of tRNA and domain IV, of elongation factor EF-G. We suggest that RRF binds to the ribosomal, A-site through its coil domain, which is a tRNA mimic. The relative, position of the two domains is changed about an axis along the hydrophobic, cleft in the hinge where the alkyl chain of a detergent molecule is bound., The tRNA mimicry and the domain movement observed in RRF provide a, structural basis for understanding the role of RRF in protein synthesis.
+
<StructureSection load='1ek8' size='340' side='right'caption='[[1ek8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ek8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EK8 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DEM:DECYLOXY-METHANOL'>DEM</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ek8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ek8 OCA], [https://pdbe.org/1ek8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ek8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ek8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ek8 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RRF_ECOLI RRF_ECOLI] Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.[HAMAP-Rule:MF_00040]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ek/1ek8_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ek8 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1EK8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with HG and DEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EK8 OCA].
+
*[[Ribosome recycling factor|Ribosome recycling factor]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure of the ribosome recycling factor from Escherichia coli., Kim KK, Min K, Suh SW, EMBO J. 2000 May 15;19(10):2362-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10811627 10811627]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Kim, K.K.]]
+
[[Category: Kim KK]]
-
[[Category: Min, K.]]
+
[[Category: Min K]]
-
[[Category: Suh, S.W.]]
+
[[Category: Suh SW]]
-
[[Category: DEM]]
+
-
[[Category: HG]]
+
-
[[Category: coiled coil]]
+
-
[[Category: ribosome]]
+
-
[[Category: t-rna mimicry]]
+
-
[[Category: translation factor]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:04:40 2007''
+

Current revision

CRYSTAL STRUCTURE OF THE RIBOSOME RECYCLING FACTOR (RRF) FROM ESCHERICHIA COLI

PDB ID 1ek8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ek8"
Personal tools