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1eke

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(New page: 200px<br /><applet load="1eke" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eke, resolution 2.00&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1eke.gif|left|200px]]<br /><applet load="1eke" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eke, resolution 2.00&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CLASS II RIBONUCLEASE H (RNASE HII) WITH MES LIGAND'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CLASS II RIBONUCLEASE H (RNASE HII) WITH MES LIGAND==
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BACKGROUND: RNases H are present in all organisms and cleave RNAs in, RNA/DNA hybrids. There are two major types of RNases H that have little, similarity in sequence, size and specificity. The structure of RNase HI, the smaller enzyme and most abundant in bacteria, has been extensively, studied. However, no structural information is available for the larger, RNase H, which is most abundant in eukaryotes and archaea. Mammalian RNase, H participates in DNA replication, removal of the Okazaki fragments and, possibly DNA repair. RESULTS: The crystal structure of RNase HII from the, hypothermophile Methanococcus jannaschii, which is homologous to mammalian, RNase H, was solved using a multiwavelength anomalous dispersion (MAD), phasing method at 2 A resolution. The structure contains two compact, domains. Despite the absence of sequence similarity, the large N-terminal, domain shares a similar fold with the RNase HI of bacteria. The active, site of RNase HII contains three aspartates: Asp7, Asp112 and Asp149. The, nucleotide-binding site is located in the cleft between the N-terminal and, C-terminal domains. CONCLUSIONS: Despite a lack of any detectable, similarity in primary structure, RNase HII shares a similar structural, domain with RNase HI, suggesting that the two classes of RNases H have a, common catalytic mechanism and possibly a common evolutionary origin. The, involvement of the unique C-terminal domain in substrate recognition, explains the different reaction specificity observed between the two, classes of RNase H.
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<StructureSection load='1eke' size='340' side='right'caption='[[1eke]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eke]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EKE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eke OCA], [https://pdbe.org/1eke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eke RCSB], [https://www.ebi.ac.uk/pdbsum/1eke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eke ProSAT], [https://www.topsan.org/Proteins/BSGC/1eke TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNH2_METJA RNH2_METJA] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.[HAMAP-Rule:MF_00052_A]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ek/1eke_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eke ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EKE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MES as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EKE OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of archaeal RNase HII: a homologue of human major RNase H., Lai L, Yokota H, Hung LW, Kim R, Kim SH, Structure. 2000 Aug 15;8(8):897-904. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10997908 10997908]
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Ribonuclease H]]
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[[Category: Hung LW]]
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[[Category: Single protein]]
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[[Category: Kim R]]
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[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
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[[Category: Kim SH]]
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[[Category: Hung, L.W.]]
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[[Category: Lai LH]]
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[[Category: Kim, R.]]
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[[Category: Yokota H]]
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[[Category: Kim, S.H.]]
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[[Category: Lai, L.H.]]
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[[Category: Yokota, H.]]
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[[Category: MES]]
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[[Category: berkeley structural genomics center]]
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[[Category: bsgc structure funded by nih]]
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[[Category: endonuclease]]
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[[Category: nuclease]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:04:48 2007''
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Current revision

CRYSTAL STRUCTURE OF CLASS II RIBONUCLEASE H (RNASE HII) WITH MES LIGAND

PDB ID 1eke

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