1em9

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(New page: 200px<br /><applet load="1em9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1em9, resolution 2.05&Aring;" /> '''ROUS SARCOMA VIRUS C...)
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[[Image:1em9.jpg|left|200px]]<br /><applet load="1em9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1em9, resolution 2.05&Aring;" />
 
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'''ROUS SARCOMA VIRUS CAPSID PROTEIN: N-TERMINAL DOMAIN'''<br />
 
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==Overview==
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==ROUS SARCOMA VIRUS CAPSID PROTEIN: N-TERMINAL DOMAIN==
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BACKGROUND: The capsid protein (CA) of retroviruses, such as Rous sarcoma, virus (RSV), consists of two independently folded domains. CA functions as, part of a polyprotein during particle assembly and budding and, in, addition, forms a shell encapsidating the genomic RNA in the mature, infectious virus. RESULTS: The structures of the N- and C-terminal domains, of RSV CA have been determined by X-ray crystallography and solution, nuclear magnetic resonance (NMR) spectroscopy, respectively. The, N-terminal domain comprises seven alpha helices and a short beta hairpin, at the N terminus. The N-terminal domain associates through a small, tightly packed, twofold symmetric interface within the crystal, different, from those previously described for other retroviral CAs. The C-terminal, domain is a compact bundle of four alpha helices, although the last few, residues are disordered. In dilute solution, RSV CA is predominantly, monomeric. We show, however, using electron microscopy, that intact RSV CA, can assemble in vitro to form both tubular structures constructed from, toroidal oligomers and planar monolayers. Both modes of assembly occur, under similar solution conditions, and both sheets and tubes exhibit, long-range order. CONCLUSIONS: The tertiary structure of CA is conserved, across the major retroviral genera, yet sequence variations are sufficient, to cause change in associative behavior. CA forms the exterior shell of, the viral core in all mature retroviruses. However, the core morphology, differs between viruses. Consistent with this observation, we find that, the capsid proteins of RSV and human immunodeficiency virus type 1 exhibit, different associative behavior in dilute solution and assemble in vitro, into different structures.
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<StructureSection load='1em9' size='340' side='right'caption='[[1em9]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1em9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rous_sarcoma_virus_-_Prague_C Rous sarcoma virus - Prague C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EM9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1em9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1em9 OCA], [https://pdbe.org/1em9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1em9 RCSB], [https://www.ebi.ac.uk/pdbsum/1em9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1em9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAG_RSVP GAG_RSVP] Capsid protein p27 forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1em9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1em9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EM9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EM9 OCA].
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*[[Gag polyprotein 3D structures|Gag polyprotein 3D structures]]
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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==Reference==
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__TOC__
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Structure and self-association of the Rous sarcoma virus capsid protein., Kingston RL, Fitzon-Ostendorp T, Eisenmesser EZ, Schatz GW, Vogt VM, Post CB, Rossmann MG, Structure. 2000 Jun 15;8(6):617-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10873863 10873863]
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</StructureSection>
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[[Category: Rous sarcoma virus]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Rous sarcoma virus - Prague C]]
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[[Category: Eisenmesser, E.Z.]]
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[[Category: Eisenmesser EZ]]
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[[Category: Fitzon-Ostendorp, T.]]
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[[Category: Fitzon-Ostendorp T]]
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[[Category: Kingston, R.L.]]
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[[Category: Kingston RL]]
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[[Category: Post, C.B.]]
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[[Category: Post CB]]
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[[Category: Rossmann, M.G.]]
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[[Category: Rossmann MG]]
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[[Category: Schatz, G.W.]]
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[[Category: Schatz GW]]
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[[Category: Vogt, V.M.]]
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[[Category: Vogt VM]]
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[[Category: MG]]
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[[Category: virus/viral protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:07:25 2007''
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Current revision

ROUS SARCOMA VIRUS CAPSID PROTEIN: N-TERMINAL DOMAIN

PDB ID 1em9

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