1eop

[[Image:1eop.gif|left|200px]]<br /><applet load="1eop" size="450" color="white" frame="true" align="right" spinBox="true"

caption="1eop, resolution 2.60&Aring;" />

'''ECORV BOUND TO COGNATE DNA'''<br />

==Overview==

Two new high-resolution cocrystal structures of EcoRV endonuclease bound, to DNA show that a large variation in DNA-bending angles is sampled in the, ground state binary complex. Together with previous structures, these data, reveal a contiguous series of protein conformational states delineating a, specific trajectory for the induced-fit pathway. Rotation of the, DNA-binding domains, together with movements of two symmetry-related, helices binding in the minor groove, causes base unstacking at a key, base-pair step and propagates structural changes that assemble the active, sites. These structures suggest a complex mechanism for DNA bending that, depends on forces generated by interacting protein segments, and on, selective neutralization of phosphate charges along the inner face of the, bent double helix.

==About this Structure==

1EOP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EOP OCA].

 

Crystallographic snapshots along a protein-induced DNA-bending pathway., Horton NC, Perona JJ, Proc Natl Acad Sci U S A. 2000 May 23;97(11):5729-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10801972 10801972]

[[Category: Single protein]]

[[Category: Type II site-specific deoxyribonuclease]]

[[Category: Horton, N.C.]]

[[Category: Perona, J.J.]]