1ce7

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{{Seed}}
 
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[[Image:1ce7.png|left|200px]]
 
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==MISTLETOE LECTIN I FROM VISCUM ALBUM==
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The line below this paragraph, containing "STRUCTURE_1ce7", creates the "Structure Box" on the page.
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<StructureSection load='1ce7' size='340' side='right'caption='[[1ce7]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ce7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CE7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CE7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_1ce7| PDB=1ce7 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ce7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ce7 OCA], [https://pdbe.org/1ce7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ce7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ce7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ce7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ML4_VISAL ML4_VISAL] The A chain is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing adenine from position 4,324 of 28S rRNA. The B chain binds to cell receptors and probably facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (lectin activity). Inhibits growth of the human tumor cell line Molt4.<ref>PMID:15001393</ref> <ref>PMID:1450445</ref> [UniProtKB:P81446]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ce/1ce7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ce7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the ribosome-inactivating protein (RIP) mistletoe lectin I (ML-I) from Viscum album has been solved by molecular replacement techniques. The structure has been refined to a crystallographic R-factor of 24.5% using X-ray diffraction data to 2.8 A resolution. The heterodimeric 63-kDa protein consists of a toxic A subunit which exhibits RNA-glycosidase activity and a galactose-specific lectin B subunit. The overall protein fold is similar to that of ricin from Ricinus communis; however, unlike ricin, ML-I is already medically applied as a component of a commercially available misteltoe extract with immunostimulating potency and for the treatment of human cancer. The three-dimensional structure reported here revealed structural details of this pharmaceutically important protein. The comparison to the structure of ricin gives more insights into the functional mechanism of this protein, provides structural details for further protein engineering studies, and may lead to the development of more effective therapeutic RIPs.
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===MISTLETOE LECTIN I FROM VISCUM ALBUM===
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Crystal structure of mistletoe lectin I from Viscum album.,Krauspenhaar R, Eschenburg S, Perbandt M, Kornilov V, Konareva N, Mikailova I, Stoeva S, Wacker R, Maier T, Singh T, Mikhailov A, Voelter W, Betzel C Biochem Biophys Res Commun. 1999 Apr 13;257(2):418-24. PMID:10198229<ref>PMID:10198229</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10198229}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1ce7" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10198229 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10198229}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1CE7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CE7 OCA].
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==Reference==
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Crystal structure of mistletoe lectin I from Viscum album., Krauspenhaar R, Eschenburg S, Perbandt M, Kornilov V, Konareva N, Mikailova I, Stoeva S, Wacker R, Maier T, Singh T, Mikhailov A, Voelter W, Betzel C, Biochem Biophys Res Commun. 1999 Apr 13;257(2):418-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10198229 10198229]
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[[Category: Single protein]]
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[[Category: Viscum album]]
[[Category: Viscum album]]
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[[Category: Betzel, C.]]
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[[Category: Betzel C]]
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[[Category: Eschenburg, S.]]
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[[Category: Eschenburg S]]
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[[Category: Konareva, N.]]
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[[Category: Konareva N]]
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[[Category: Kornilov, V.]]
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[[Category: Kornilov V]]
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[[Category: Krauspenhaar, R.]]
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[[Category: Krauspenhaar R]]
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[[Category: Maier, T.]]
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[[Category: Maier T]]
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[[Category: Mikailova, I.]]
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[[Category: Mikailova I]]
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[[Category: Mikhailov, A.]]
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[[Category: Mikhailov A]]
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[[Category: Perbandt, M.]]
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[[Category: Perbandt M]]
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[[Category: Singh, T P.]]
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[[Category: Singh TP]]
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[[Category: Stoeva, S.]]
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[[Category: Stoeva S]]
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[[Category: Voelter, W.]]
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[[Category: Voelter W]]
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[[Category: Wacker, R.]]
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[[Category: Wacker R]]
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[[Category: Ribosome-inactivating protein type ii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:37:25 2008''
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Current revision

MISTLETOE LECTIN I FROM VISCUM ALBUM

PDB ID 1ce7

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