1cf5

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{{Seed}}
 
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[[Image:1cf5.png|left|200px]]
 
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==BETA-MOMORCHARIN STRUCTURE AT 2.55 A==
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The line below this paragraph, containing "STRUCTURE_1cf5", creates the "Structure Box" on the page.
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<StructureSection load='1cf5' size='340' side='right'caption='[[1cf5]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1cf5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CF5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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{{STRUCTURE_1cf5| PDB=1cf5 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cf5 OCA], [https://pdbe.org/1cf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cf5 RCSB], [https://www.ebi.ac.uk/pdbsum/1cf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cf5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIP3_MOMCH RIP3_MOMCH] Irreversibly relaxes supercoiled DNA and catalyzes double-stranded breakage. Acts also as a ribosome inactivating protein.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cf5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cf5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Beta-Momorcharin (Mr approximately 29 kDa) is a single-chained ribosome-inactivating protein (RIP) with a branched hexasaccharide bound to Asn51. The crystal structure of beta-momorcharin has been determined using the molecular-replacement method and refined to 2. 55 A resolution. The final structural model gave an R factor of 17. 2% and root-mean-square deviations of 0.016 A and 1.76 degrees from ideal bond lengths and bond angles, respectively. beta-Momorcharin contains nine alpha-helices, two 310 helices and three beta-sheets, and its overall structure is similar to those of other single-chained RIPs. Residues Tyr70, Tyr109, Glu158 and Arg161 are expected to define the active site of beta-momorcharin as an rRNA N-glycosidase. The oligosaccharide is linked to the protein through an N-glycosidic bond, beta-GlcNAc-(1-N)-Asn51, and stretches from the surface of the N-terminal domain far from the active site, which suggests that it should not play a role in enzymatic function. The oligosaccharide of each beta-momorcharin molecule interacts with the protein through hydrogen bonds, although in the crystals most of these are intermolecular interactions with the protein atoms in an adjacent unit cell. This is the first example of an RIP structure which provides information about the three-dimensional structure and binding site of the oligosaccharide in the active chains of RIPs.
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===BETA-MOMORCHARIN STRUCTURE AT 2.55 A===
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Three-dimensional structure of beta-momorcharin at 2.55 A resolution.,Yuan YR, He YN, Xiong JP, Xia ZX Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1144-51. PMID:10329776<ref>PMID:10329776</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1cf5" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10329776}}, adds the Publication Abstract to the page
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10329776 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10329776}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1CF5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CF5 OCA].
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==Reference==
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Three-dimensional structure of beta-momorcharin at 2.55 A resolution., Yuan YR, He YN, Xiong JP, Xia ZX, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1144-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10329776 10329776]
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[[Category: Momordica charantia]]
[[Category: Momordica charantia]]
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[[Category: Single protein]]
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[[Category: He Y-N]]
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[[Category: He, Y N.]]
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[[Category: Xia Z-X]]
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[[Category: Xia, Z X.]]
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[[Category: Xiong J-P]]
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[[Category: Xiong, J P.]]
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[[Category: Yuan Y-R]]
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[[Category: Yuan, Y R.]]
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[[Category: Glycoprotein]]
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[[Category: Ribosome-inactivating protein]]
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[[Category: Rna n-glycosidase activity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 20:39:57 2008''
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Current revision

BETA-MOMORCHARIN STRUCTURE AT 2.55 A

PDB ID 1cf5

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