1etj

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(New page: 200px<br /><applet load="1etj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1etj, resolution 2.3&Aring;" /> '''AZURIN MUTANT WITH ME...)
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[[Image:1etj.gif|left|200px]]<br /><applet load="1etj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1etj, resolution 2.3&Aring;" />
 
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'''AZURIN MUTANT WITH MET 121 REPLACED BY GLU'''<br />
 
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==Overview==
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==AZURIN MUTANT WITH MET 121 REPLACED BY GLU==
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The Met121Glu azurin mutant has been crystallized and the structure, determined at a resolution of 2.3 A. In the crystal structure a carboxyl, oxygen of Met121Glu is coordinated to the metal at a distance of 2.2 A., Single-crystal resonance Raman spectroscopy was used to show that the, glutamic acid residue in the copper site was in the protonated state., Titration of this residue gives rise to a number of unusual, pH-dependent, properties: as the pH is increased from 4 to 8, the S(Cys)-Cu, ligand-to-metal charge transfer bands are blue shifted and their intensity, ratio is reversed, the EPR signal changes from type 1 copper to a new form, of protein-bound copper, and the redox potential changes from 370 to 180, mV. The spectroscopic changes in this pH interval are consistent with a, two-state model. From the pH dependence of the optical and EPR spectra, pKa = 5.0 for the glutamic acid in the oxidized protein was determined.
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<StructureSection load='1etj' size='340' side='right'caption='[[1etj]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1etj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ETJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1etj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etj OCA], [https://pdbe.org/1etj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1etj RCSB], [https://www.ebi.ac.uk/pdbsum/1etj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1etj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/et/1etj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1etj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Met121Glu azurin mutant has been crystallized and the structure determined at a resolution of 2.3 A. In the crystal structure a carboxyl oxygen of Met121Glu is coordinated to the metal at a distance of 2.2 A. Single-crystal resonance Raman spectroscopy was used to show that the glutamic acid residue in the copper site was in the protonated state. Titration of this residue gives rise to a number of unusual, pH-dependent properties: as the pH is increased from 4 to 8, the S(Cys)-Cu ligand-to-metal charge transfer bands are blue shifted and their intensity ratio is reversed, the EPR signal changes from type 1 copper to a new form of protein-bound copper, and the redox potential changes from 370 to 180 mV. The spectroscopic changes in this pH interval are consistent with a two-state model. From the pH dependence of the optical and EPR spectra, pKa = 5.0 for the glutamic acid in the oxidized protein was determined.
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==About this Structure==
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X-ray structure determination and characterization of the Pseudomonas aeruginosa azurin mutant Met121Glu.,Karlsson BG, Tsai LC, Nar H, Sanders-Loehr J, Bonander N, Langer V, Sjolin L Biochemistry. 1997 Apr 8;36(14):4089-95. PMID:9100002<ref>PMID:9100002</ref>
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1ETJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ETJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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X-ray structure determination and characterization of the Pseudomonas aeruginosa azurin mutant Met121Glu., Karlsson BG, Tsai LC, Nar H, Sanders-Loehr J, Bonander N, Langer V, Sjolin L, Biochemistry. 1997 Apr 8;36(14):4089-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9100002 9100002]
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</div>
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[[Category: Pseudomonas aeruginosa]]
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<div class="pdbe-citations 1etj" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Bonander, N.]]
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[[Category: Karlsson, B.G.]]
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[[Category: Langer, V.]]
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[[Category: Nar, H.]]
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[[Category: Sanders-Loehr, J.]]
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[[Category: Sjolin, L.]]
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[[Category: Tsai, L.C.]]
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[[Category: CU]]
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[[Category: copper]]
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[[Category: electron transport]]
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[[Category: periplasmic]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:18:04 2007''
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==See Also==
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*[[Azurin 3D structures|Azurin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Bonander N]]
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[[Category: Karlsson BG]]
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[[Category: Langer V]]
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[[Category: Nar H]]
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[[Category: Sanders-Loehr J]]
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[[Category: Sjolin L]]
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[[Category: Tsai L-C]]

Current revision

AZURIN MUTANT WITH MET 121 REPLACED BY GLU

PDB ID 1etj

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