1cu4

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{{Seed}}
 
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[[Image:1cu4.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF THE ANTI-PRION FAB 3F4 IN COMPLEX WITH ITS PEPTIDE EPITOPE==
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The line below this paragraph, containing "STRUCTURE_1cu4", creates the "Structure Box" on the page.
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<StructureSection load='1cu4' size='340' side='right'caption='[[1cu4]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1cu4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CU4 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cu4 OCA], [https://pdbe.org/1cu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cu4 RCSB], [https://www.ebi.ac.uk/pdbsum/1cu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cu4 ProSAT]</span></td></tr>
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{{STRUCTURE_1cu4| PDB=1cu4 | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cu4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cu4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The X-ray crystallographic structures of the anti-Syrian hamster prion protein (SHaPrP) monoclonal Fab 3F4 alone, as well as the complex with its cognate peptide epitope (SHaPrP 104-113), have been determined to atomic resolution. The conformation of the decapeptide is an Omega-loop. There are substantial alterations in the antibody combining region upon epitope binding. The peptide binds in a U-shaped groove on the Fab surface, with the two specificity determinants, Met109 and Met112, penetrating deeply into separate hydrophobic cavities formed by the heavy and light chain complementarity-determining regions. In addition to the numerous contacts between the Fab and the peptide, two intrapeptide hydrogen bonds are observed, perhaps indicating the structure bound to the Fab exists transiently in solution. This provides the first structural information on a portion of the PrP N-terminal region observed to be flexible in the NMR studies of SHPrP 90-231, SHaPrP 29-231 and mouse PrP 23-231. Antibody characterization of the antigenic surfaces of PrPC and PrPSc identifies this flexible region as a component of the conformational rearrangement that is an essential feature of prion disease.
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===CRYSTAL STRUCTURE OF THE ANTI-PRION FAB 3F4 IN COMPLEX WITH ITS PEPTIDE EPITOPE===
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Antibody binding defines a structure for an epitope that participates in the PrPC--&gt;PrPSc conformational change.,Kanyo ZF, Pan KM, Williamson RA, Burton DR, Prusiner SB, Fletterick RJ, Cohen FE J Mol Biol. 1999 Nov 5;293(4):855-63. PMID:10543972<ref>PMID:10543972</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1cu4" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10543972 is the PubMed ID number.
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*[[Prion 3D structures|Prion 3D structures]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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{{ABSTRACT_PUBMED_10543972}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CU4 OCA].
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</StructureSection>
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[[Category: Large Structures]]
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==Reference==
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[[Category: Mus musculus]]
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Antibody binding defines a structure for an epitope that participates in the PrPC--&gt;PrPSc conformational change., Kanyo ZF, Pan KM, Williamson RA, Burton DR, Prusiner SB, Fletterick RJ, Cohen FE, J Mol Biol. 1999 Nov 5;293(4):855-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10543972 10543972]
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[[Category: Burton DR]]
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[[Category: Burton, D R.]]
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[[Category: Cohen FE]]
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[[Category: Cohen, F E.]]
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[[Category: Fletterick RJ]]
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[[Category: Fletterick, R J.]]
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[[Category: Kanyo ZF]]
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[[Category: Kanyo, Z F.]]
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[[Category: Pan KM]]
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[[Category: Pan, K M.]]
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[[Category: Prusiner SB]]
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[[Category: Prusiner, S B.]]
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[[Category: Williamson RA]]
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[[Category: Williamson, R A.]]
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[[Category: Immunoglobulin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 21:22:23 2008''
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Current revision

CRYSTAL STRUCTURE OF THE ANTI-PRION FAB 3F4 IN COMPLEX WITH ITS PEPTIDE EPITOPE

PDB ID 1cu4

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