1d6k

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{{Seed}}
 
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[[Image:1d6k.png|left|200px]]
 
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==NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX==
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The line below this paragraph, containing "STRUCTURE_1d6k", creates the "Structure Box" on the page.
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<StructureSection load='1d6k' size='340' side='right'caption='[[1d6k]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1d6k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D6K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d6k OCA], [https://pdbe.org/1d6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d6k RCSB], [https://www.ebi.ac.uk/pdbsum/1d6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d6k ProSAT]</span></td></tr>
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{{STRUCTURE_1d6k| PDB=1d6k | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL25_ECOLI RL25_ECOLI] This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance. Binds to the 5S rRNA independently of L5 and L18. Not required for binding of the 5S rRNA/L5/L18 subcomplex to 23S rRNA.[HAMAP-Rule:MF_01336]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d6/1d6k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d6k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the complex between ribosomal protein L25 and a 37 nucleotide RNA molecule, which contains the E-loop and helix IV regions of the E-domain of Escherichia coli 5S rRNA, has been determined to an overall r.m.s. displacement of 1.08 A (backbone heavy atoms) by heteronuclear NMR spectroscopy (Protein Databank code 1d6k). The interacting molecular surfaces are bipartite for both the RNA and the protein. One side of the six-stranded beta-barrel of L25 recognizes the minor groove of the E-loop with very little change in the conformations of either the protein or the RNA and with the RNA-protein interactions occurring mainly along one strand of the E-loop duplex. This minor groove recognition module includes two parallel beta-strands of L25, a hitherto unknown RNA binding topology. Binding of the RNA also induces conversion of a flexible loop to an alpha-helix in L25, the N-terminal tip of which interacts with the widened major groove at the E-loop/helix IV junction of the RNA. The structure of the complex reveals that the E-domain RNA serves as a preformed docking partner, while the L25 protein has one preformed and one induced recognition module.
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===NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX===
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The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition.,Stoldt M, Wohnert J, Ohlenschlager O, Gorlach M, Brown LR EMBO J. 1999 Nov 15;18(22):6508-21. PMID:10562563<ref>PMID:10562563</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1d6k" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10562563}}, adds the Publication Abstract to the page
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*[[Ribosomal protein L25|Ribosomal protein L25]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10562563 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10562563}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1D6K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D6K OCA].
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==Reference==
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The NMR structure of the 5S rRNA E-domain-protein L25 complex shows preformed and induced recognition., Stoldt M, Wohnert J, Ohlenschlager O, Gorlach M, Brown LR, EMBO J. 1999 Nov 15;18(22):6508-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10562563 10562563]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brown, L R.]]
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[[Category: Brown LR]]
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[[Category: Gorlach, M.]]
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[[Category: Gorlach M]]
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[[Category: Ohlenschlager, O.]]
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[[Category: Ohlenschlager O]]
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[[Category: Stoldt, M.]]
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[[Category: Stoldt M]]
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[[Category: Wohnert, J.]]
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[[Category: Wohnert J]]
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[[Category: Protein-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:32:26 2008''
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Current revision

NMR SOLUTION STRUCTURE OF THE 5S RRNA E-LOOP/L25 COMPLEX

PDB ID 1d6k

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