1eyu

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(New page: 200px<br /><applet load="1eyu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eyu, resolution 1.78&Aring;" /> '''HIGH RESOLUTION STRU...)
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[[Image:1eyu.gif|left|200px]]<br /><applet load="1eyu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eyu, resolution 1.78&Aring;" />
 
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'''HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6'''<br />
 
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==Overview==
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==HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6==
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Restriction endonucleases differ in their use of metal cofactors despite, having remarkably similar folds for their catalytic regions. To explore, this, we have characterized the interaction of endonuclease PvuII with the, catalytically incompetent cation Ca(2+). The structure of a, glutaraldehyde-crosslinked crystal of the endonuclease PvuII-DNA complex, determined in the presence of Ca(2+) at a pH of approximately 6.5, supports a two-metal mechanism of DNA cleavage by PvuII. The first Ca(2+), position matches that found in all structurally examined endonucleases, while the second position is similar to that of EcoRV but is distinct from, that of BamHI and BglI. The location of the second metal in PvuII, unlike, that in BamHI/BglI, permits no direct interaction between the second metal, and the O3' oxygen leaving group. However, the interactions between the, DNA scissile phosphate and the metals, the first metal and the attacking, water, and the attacking water and DNA are the same in PvuII as they are, in the two-metal models of BamHI and BglI, but are distinct from the, proposed three-metal or the two-metal models of EcoRV.
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<StructureSection load='1eyu' size='340' side='right'caption='[[1eyu]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eyu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyu OCA], [https://pdbe.org/1eyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyu RCSB], [https://www.ebi.ac.uk/pdbsum/1eyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/T2P2_PROHU T2P2_PROHU] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3.
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==About this Structure==
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==See Also==
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1EYU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EYU OCA].
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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PvuII endonuclease contains two calcium ions in active sites., Horton JR, Cheng X, J Mol Biol. 2000 Jul 28;300(5):1049-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10903853 10903853]
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[[Category: Large Structures]]
[[Category: Proteus vulgaris]]
[[Category: Proteus vulgaris]]
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[[Category: Single protein]]
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[[Category: Cheng X]]
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[[Category: Type II site-specific deoxyribonuclease]]
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[[Category: Horton JR]]
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[[Category: Cheng, X.]]
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[[Category: Horton, J.R.]]
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[[Category: endonuclease type ii]]
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[[Category: protein-dna complex]]
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[[Category: restriction enzyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:26:42 2007''
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Current revision

HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6

PDB ID 1eyu

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