1eyv

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(New page: 200px<br /><applet load="1eyv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eyv, resolution 1.6&Aring;" /> '''THE CRYSTAL STRUCTURE...)
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[[Image:1eyv.gif|left|200px]]<br /><applet load="1eyv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eyv, resolution 1.6&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF NUSB FROM MYCOBACTERIUM TUBERCULOSIS'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF NUSB FROM MYCOBACTERIUM TUBERCULOSIS==
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Both prokaryotes and eukaryotes regulate transcription through mechanisms, that suppress termination signals. An antitermination mechanism was first, characterized in bacteriophage lambda. Bacteria have analogous machinery, that regulates ribosomal RNA transcription and employs host factors, called the N-utilizing (where N stands for the phage lambda N protein), substances (Nus), NusA, NusB, NusE and NusG. Here we report the crystal, structure of NusB from Mycobacterium tuberculosis, the bacterium that, causes tuberculosis in humans. This molecule shares a similar tertiary, structure with the related Escherichia coli protein but adopts a different, quaternary organization. We show that, unlike the E. coli homolog, M., tuberculosis NusB is dimeric both in solution and in the crystal. These, data help provide a framework for understanding the structural and, biological function of NusB in the prokaryotic transcriptional, antitermination complex.
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<StructureSection load='1eyv' size='340' side='right'caption='[[1eyv]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1eyv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EYV FirstGlance]. <br>
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1EYV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EYV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eyv OCA], [https://pdbe.org/1eyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eyv RCSB], [https://www.ebi.ac.uk/pdbsum/1eyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eyv ProSAT], [https://www.topsan.org/Proteins/TBSGC/1eyv TOPSAN]</span></td></tr>
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The crystal structure of NusB from Mycobacterium tuberculosis., Gopal B, Haire LF, Cox RA, Jo Colston M, Major S, Brannigan JA, Smerdon SJ, Dodson G, Nat Struct Biol. 2000 Jun;7(6):475-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10881194 10881194]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NUSB_MYCTU NUSB_MYCTU] Involved in the transcription termination process (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ey/1eyv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eyv ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Brannigan JA]]
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[[Category: Brannigan, J.A.]]
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[[Category: Colston MJ]]
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[[Category: Colston, M.J.]]
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[[Category: Cox RA]]
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[[Category: Cox, R.A.]]
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[[Category: Dodson GG]]
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[[Category: Dodson, G.G.]]
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[[Category: Gopal B]]
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[[Category: Gopal, B.]]
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[[Category: Haire LF]]
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[[Category: Haire, L.F.]]
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[[Category: Major S]]
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[[Category: Major, S.]]
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[[Category: Smerdon SJ]]
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[[Category: Smerdon, S.J.]]
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[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
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[[Category: PO4]]
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[[Category: helical bundle]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:26:45 2007''
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Current revision

THE CRYSTAL STRUCTURE OF NUSB FROM MYCOBACTERIUM TUBERCULOSIS

PDB ID 1eyv

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