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1ezm

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(New page: 200px<br /><applet load="1ezm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ezm, resolution 1.5&Aring;" /> '''THREE-DIMENSIONAL STR...)
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[[Image:1ezm.gif|left|200px]]<br /><applet load="1ezm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ezm, resolution 1.5&Aring;" />
 
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'''THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION==
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Pseudomonas aeruginosa elastase (PAE) is a zinc metalloprotease with 301, amino acids. We have crystallized and solved the three-dimensional, structure of PAE, using data to 1.5-A resolution, and have refined the, native molecular structure to R = 0.188. The overall tertiary structure of, the PAE molecule is similar to that of thermolysin, with which it shares, 28% amino acid sequence identity. Nearly all of the active site residues, that might potentially interact with substrates are identical in the two, proteins. However, the active site cleft is significantly more "open" in, PAE than in thermolysin.
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<StructureSection load='1ezm' size='340' side='right'caption='[[1ezm]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ezm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EZM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ezm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezm OCA], [https://pdbe.org/1ezm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ezm RCSB], [https://www.ebi.ac.uk/pdbsum/1ezm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ezm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ELAS_PSEAE ELAS_PSEAE] Cleaves elastin, collagen, IgG, and several complement components. Involved in the pathogenesis of P.aeruginosa infections.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/1ezm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ezm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EZM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ZN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pseudolysin Pseudolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.26 3.4.24.26] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EZM OCA].
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*[[Elastase 3D structures|Elastase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Three-dimensional structure of the elastase of Pseudomonas aeruginosa at 1.5-A resolution., Thayer MM, Flaherty KM, McKay DB, J Biol Chem. 1991 Feb 15;266(5):2864-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1899664 1899664]
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[[Category: Large Structures]]
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[[Category: Pseudolysin]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Flaherty KM]]
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[[Category: Flaherty, K.M.]]
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[[Category: Mckay DB]]
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[[Category: Mckay, D.B.]]
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[[Category: Thayer MM]]
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[[Category: Thayer, M.M.]]
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[[Category: CA]]
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[[Category: ZN]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:28:13 2007''
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Current revision

THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION

PDB ID 1ezm

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