1f08

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(New page: 200px<br /><applet load="1f08" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f08, resolution 1.90&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1f08.gif|left|200px]]<br /><applet load="1f08" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f08, resolution 1.90&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS==
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Papillomaviral infection causes both benign and malignant lesions and is a, necessary cause of cervical carcinoma. Replication of this virus requires, the replication initiation proteins E1 and E2, which bind cooperatively at, the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a, precursor to larger E1 complexes that distort and unwind the ori. We, present the crystal structure of the E1 DNA binding domain refined to 1.9, A resolution. Residues critical for DNA binding are located on an extended, loop and an alpha helix. We identify the E1 dimerization surface by, selective mutations at an E1/E1 interface observed in the crystal and, propose a model for the (E1)2-DNA complex. These and other observations, suggest how the E1 DNA binding domain orchestrates assembly of the, hexameric helicase on the ori.
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<StructureSection load='1f08' size='340' side='right'caption='[[1f08]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f08]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F08 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f08 OCA], [https://pdbe.org/1f08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f08 RCSB], [https://www.ebi.ac.uk/pdbsum/1f08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f08 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VE1_BPV1 VE1_BPV1] ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f0/1f08_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f08 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1F08 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus] with BR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F08 OCA].
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*[[Replication protein E1|Replication protein E1]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus., Enemark EJ, Chen G, Vaughn DE, Stenlund A, Joshua-Tor L, Mol Cell. 2000 Jul;6(1):149-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10949036 10949036]
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[[Category: Bovine papillomavirus]]
[[Category: Bovine papillomavirus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, G.]]
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[[Category: Chen G]]
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[[Category: Enemark, E.J.]]
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[[Category: Enemark EJ]]
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[[Category: Joshua-Tor, L.]]
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[[Category: Joshua-Tor L]]
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[[Category: Stenlund, A.]]
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[[Category: Stenlund A]]
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[[Category: Vaughn, D.E.]]
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[[Category: Vaughn DE]]
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[[Category: BR]]
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[[Category: dna-binding domain]]
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[[Category: helicase]]
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[[Category: initiator protein]]
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[[Category: papillomavirus]]
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[[Category: replication]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:29:28 2007''
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Current revision

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS

PDB ID 1f08

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