1f1z

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(New page: 200px<br /><applet load="1f1z" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f1z, resolution 2.40&Aring;" /> '''TNSA, a catalytic co...)
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[[Image:1f1z.gif|left|200px]]<br /><applet load="1f1z" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f1z, resolution 2.40&Aring;" />
 
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'''TNSA, a catalytic component of the TN7 transposition system'''<br />
 
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==Overview==
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==TNSA, a catalytic component of the TN7 transposition system==
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Transposition requires a coordinated series of DNA breakage and joining, reactions. The Tn7 transposase contains two proteins: TnsA, which carries, out DNA breakage at the 5' ends of the transposon, and TnsB, which carries, out breakage and joining at the 3' ends of the transposon. TnsB is a, member of the retroviral integrase superfamily whose hallmark is a, conserved DDE motif. We report here the structure of TnsA at 2.4 A, resolution. Surprisingly, the TnsA fold is that of a type II restriction, endonuclease. Thus, Tn7 transposition involves a collaboration between, polypeptides, one containing a DDE motif and one that does not. This, result indicates that the range of biological processes that utilize, restriction enzyme-like folds also includes DNA transposition.
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<StructureSection load='1f1z' size='340' side='right'caption='[[1f1z]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f1z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F1Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f1z OCA], [https://pdbe.org/1f1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f1z RCSB], [https://www.ebi.ac.uk/pdbsum/1f1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f1z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TNSA_ECOLX TNSA_ECOLX] Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and TnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas TnsABC and TnsD promote low-frequency insertion into many different sites.<ref>PMID:8947057</ref> <ref>PMID:10704304</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f1/1f1z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f1z ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1F1Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F1Z OCA].
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*[[Transposase 3D structures|Transposase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Unexpected structural diversity in DNA recombination: the restriction endonuclease connection., Hickman AB, Li Y, Mathew SV, May EW, Craig NL, Dyda F, Mol Cell. 2000 Jun;5(6):1025-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10911996 10911996]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Craig, N.L.]]
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[[Category: Craig NL]]
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[[Category: Dyda, F.]]
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[[Category: Dyda F]]
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[[Category: Hickman, A.B.]]
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[[Category: Hickman AB]]
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[[Category: Li, Y.]]
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[[Category: Li Y]]
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[[Category: Mathew, S.V.]]
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[[Category: Mathew SV]]
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[[Category: May, E.W.]]
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[[Category: May EW]]
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[[Category: CL]]
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[[Category: MG]]
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[[Category: restriction endonuclease fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:31:54 2007''
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Current revision

TNSA, a catalytic component of the TN7 transposition system

PDB ID 1f1z

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