1f3g

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(New page: 200px<br /><applet load="1f3g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f3g, resolution 2.1&Aring;" /> '''THREE-DIMENSIONAL STR...)
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[[Image:1f3g.jpg|left|200px]]<br /><applet load="1f3g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f3g, resolution 2.1&Aring;" />
 
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'''THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC'''<br />
 
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==Overview==
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==THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC==
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The crystal structure of a proteolytically modified form of the, Escherichia coli phosphocarrier and signal transducing protein IIIglc has, been determined by multiple isomorphous and molecular replacement. The, model has been refined to an R-factor of 0.166 for data between 6- and, 2.1-A resolution with an rms deviation of 0.020 A from ideal bond lengths, and 3.2 degrees from ideal bond angles. The molecule is a beta-sheet, sandwich, with six antiparallel strands on either side. Several short, distorted helices line the periphery of the active site, which is a, shallow extremely hydrophobic depression approximately 18 A in diameter, near the center of one face. The side chains of the active site histidine, residues 75 and 90 face each other at the center of the depression, with, the N3 positions exposed to solvent, separated by 3.3 A in an excellent, position to form adducts with phosphate. Chloroplatinate forms a divalent, adduct with both histidyl side chains, suggesting that the phosphodonor, reaction might proceed through a similar transition state. The hydrophobic, patch forms the primary crystal contact, suggesting a mode of association, of IIIglc with other components of the phosphoenolpyruvate-dependent, phosphotransferase system.
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<StructureSection load='1f3g' size='340' side='right'caption='[[1f3g]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f3g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F3G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f3g OCA], [https://pdbe.org/1f3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f3g RCSB], [https://www.ebi.ac.uk/pdbsum/1f3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f3g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTGA_ECOLI PTGA_ECOLI] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/1f3g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f3g ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1F3G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F3G OCA].
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*[[Phosphocarrier protein HPr 3D structures|Phosphocarrier protein HPr 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Three-dimensional structure of the Escherichia coli phosphocarrier protein IIIglc., Worthylake D, Meadow ND, Roseman S, Liao DI, Herzberg O, Remington SJ, Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10382-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1961703 1961703]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Herzberg, O.]]
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[[Category: Herzberg O]]
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[[Category: Liao, D.I.]]
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[[Category: Liao D-I]]
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[[Category: Meadow, N.]]
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[[Category: Meadow N]]
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[[Category: Remington, S.J.]]
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[[Category: Remington SJ]]
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[[Category: Roseman, S.]]
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[[Category: Roseman S]]
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[[Category: Worthylake, D.]]
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[[Category: Worthylake D]]
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[[Category: phosphotransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:34:48 2007''
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Current revision

THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC

PDB ID 1f3g

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